%PDF-1.4 % 1 0 obj<>/ProcSet[/PDF/Text/ImageB]/ExtGState<>>>/Type/Page>> endobj 4 0 obj<> endobj 5 0 obj<> endobj 6 0 obj<> endobj 7 0 obj<> endobj 8 0 obj<> endobj 9 0 obj<> endobj 10 0 obj<> endobj 11 0 obj<>stream AdobedC         #"""#''''''''''  " s!1AQa"q2B#R3b$r%C4Scs5D'6Tdt& EFVU(eufv7GWgw8HXhx)9IYiy*:JZjz!?~b`w`y2>_<7HfrDBmp@F Ӷz6lٳf͛6lٳf͛6lٳf͛6lٳfٳf͛6lٳf͛6lٳf͛6lٳf͛6l34;,R@Da[i#\mu%w˚U͵~(F$ZJMy':7)%׏0&lٳf͛6lٳf͛6lٳf͛6lٳfٳf͛6lٳf͛6lٳf͛6lٳf͛6lӟjߕ~~󶥩1}[J0*!z>ǶKu\ѯyR;$iSRM2?o[ iWZD8&(GPA6lٳf͛6lٳf͛6lٳf͛6lٳgٳf͛6lٳf͛6lٳf͛6lٳf͛6l:ޮu;HҮ!ս\gQUe$׶oyv,K56;~[3PrwY?t3[W,FjcFy \ߣ<W?oўhW74?AOg? UNU:kQ֟>+OGbݑ-ߢ<Sm4J{5\41zZ⯏+8:?Fy \ki^ie y,cVB3.Q^~csԳ}#_1IHNobODs~LR%ӛGb-ߢ<Sm4/Ko0<]%;[8+˗j~_Mwi$jZ<ٳf͟ٳf͛6lٳf͛6lٳf͛6lٳf͛6lh͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6l7hͅԣ|Yin.ᰁ% z*@=˺Viwl$KQk9cv9=F P\s_yWEiV}R٭#ckhY+J/#zn50̺n/O+Ψ^[{*"d۹ΛYjVz֭[_\W)W#T=?pn,Kk{]JBUȣQrs[/)qiG&8[8N*__}zXl1~Zkז,7Vd+X敨5GG=XIZ~%ۯ>EO{Pk;ve[ߡGM.mŮF5]7]kH[mϦW-eehwM]6 kZo4ѧye3zo4~7:Ԗzܶ:͚^kQGpIa?uL^ݵ!-ü1(k~s_,k][2Kx+=Z!j|Cڥ~a<æy]x/.G؎xHC$/:!ҿ/_4/ўG]ȒGF}lOʺM֓%ƣGZ?R9cE +|&0kb||_hvrj:>KdX'D%y=qڍ۟m//Bv1N(mrN0ޏWꏣEek$jZ!Y"{~ -~^,qXxD. l*xhUJE{4xI$nڕf9C״?cJ[x5M&%=xV(X&Fj|i2ţ].mgii [5yIvνw4Y#'pdT;R֟e06lٳgٳf͛6lٳf͛6#w{ga he49O,N9$68ٳf͔XVz^lٳbW76p=oo$ҰDQhn!ETIdtaYYv 7hf͛6lٳf͛6lٳf͛ >ܲ0E4ݚ̈́^H7δ)f͛?ٳf͛6lٳf͛6F ~{۽;Δ&KEydOL,&݉d~5{ ZyE1krOݖ 6pNlooe}DQFy ՠ?5}tsZ疴9uPiG`B2l|.̖ab[}WSM6O4<:ឡho/<^CηmjZ Ώ}Ie<K(u䒅a tB_+^Ou.b'["*8 S^ڔMu8k<ͨ6aҞaj fN*o#y1f{K+ƒ-@7`@8/@ti9z-BIV~n&k$f?qU߰g^Ϙt_#\ZΞu-_SHhZ?˔~~$S~cNҵm\WOMđ@ʱ@4>?3ɞn&kXݳҭ4ډ<7؅hNGBɾ{טt7̚_]ZmG1^ eqk=N3?h:tOg!Z{I师JxTyTEN|j7ji tNRXRUN#yΚM}$mh4 ӭ%Uğ/}'1X[k۶Ki,ߑi*F,4o֞#<kR]zV}gU{T[ G$8ѳwo:^dӴ2k6Qʷz.%)KŸޣN<_25=!=A}>ᑅH' gy񼯤iXǥ[jSMXg2zS}ZSlOE]oC{E֖~_d]2JŨogTVy3U֬m{-Eb.S=w]+a;f2Fli(a' ?kڔ)0ۢhz=luȴ;-T۩kxuXP}>j}zWTO.dN Mwo~pJO.uSO) ډ-t8^vTzWnLZ^Ėssn1euoGJ3Wǹ͗.{-ڙ|y}',%?ڭp,d}}MM:0o>%DFHW _7X_][YAeռHɑ8E2 Pksgu5_. G@MKPcyd-SU۵r7m/%VWLO,w1m$ǐv-O^o󞧯y-? Ӵ-;̪Y7EPo2|yAm5 ͛6lٳf͛6lٳf͛6F|3\iwZV̀u2#VR?Ϯ-O(iQ幞VoR{vF}m)3">q=. 8%Yⓒޚ֘{ib'/C#-MN~PiIjShwi};#O[/պS|.ך6,qOah(-[UAnaaCMjQjV7|$q\;q:n;韗zZ&DWX1D#QFq; +=sKIkGo30nUy +Eï_g [KȯFhBǮAO-וwI-ǰꅠe8%uNZ³gڱ紳(5;=;<͠OMVYzB~Z閗u֩jqhe쯧I!Ƞq$v+<&7hO+ZA ioIn=5F0qQ@w.DL֬.ony}w{jVOՍL1+Z-}7PӵMeҠmҬhvPOAo?'z]j)aNג]qK`4qp[S)RKg].y/ץN` ~EYhk .+|u}usB ;}}Arc⡌^Uf.2SiVΓy][[^_L`)C/Eo.=%ff, >x05זo -V8K%T1,lKmBkR]^$ig1FCƈUP_m;~iZG-[yo"qlf{FKZnwte`y+qJs[o5[.u`֘˨i\dZ y + |z&o_gN_Dn=Z嶃j7ZOicr`bArm𑆰OF-<M啈ҭ\KlWZ`` oPԯ:tVmfP[3]R堆Gb5S mk4Wv6JEc%5N# ȸ[KI"IVq6=)~YhWrS[h(S_F;W%:^cVAOI'ĝ#P/:L͛6lٳf͛6lٳf͛6lٳf͛6A|j/4y\w7WH CGtie;.?k'Ѥ0x2PA;6lٳf͟7w-ڟӱ_0yBO݋qp;xYea +~~"jY,/ ӑ_~z<+۝?I6 a!:oo">dQt輥yƧ$o#ʷU'Kg:mwe+2J> OF~ZטgVYJX㑕CJ-BIYÊo/ZMntpׅdg?gh^vט/?yb9CqQ#¡MtZ__uF.P&{dya(n `TTr߶v6w>W_-w"6haT_MYP)nu5IO+iΠȳXwr8FHW" V-[Pӭ.|Κ|[M-̶HwMu۫[L-n ^ɧ,}?~Roy&J/]{?#YXZj~,vz3,/#^F>MAۮ2[M+˧ڀŻޛq `ա\m目iC6\^y3Fx78L c鶾X|ժחYsgorBN^oZG59GwC sdTҊz`=?KɺՍSƥ &Ml(LP{:f}yZӴxa1Vb%F!K0kd֍"/^\!H67Sc co~4'&tT5͠Sŏk |׵zM-%&m'JhV1ZO!Ӽ-O[g.l5[ƶIuuZW?>b.jԡ֓I#Օ-ᶳُ+]/9æ5䳢мۤz4ڱ{չܴڧsH?̶kmIPB qG#AhN<~\]Η,s_BI/Ҙm?*j5l,5]6,Xg$,~XKsk:_m}Gx'(%gXZ_jx͛6l ;+y3H#uq >͛?ٳf͛6ٳ͛6ٳ͛6Ҽ7u)5μZš,4ꚰ/2־?ƛ}ܰ4/ϲ؎Yf͛66k4n+$ԯ;Ս^c+7j*.1ߧ5˴܏Z˧5^>۾ؚrհ®׳޹$z79M,E7Գ?S޹$ֵJnWo_o0Q_.z]Xj=O8ĿM,Ev'ўM37v=VmO&!vԯco\j,50εp5՝Γmwe綝9ԫUYYy hWmxT; o#d~$Rm5f//do71Ի'%YK?.j__.IvVGtUF6)m DccQ5툥Yd}ڜY_ZjVL$hU f͟ٳf͛6lٳf͛6lٳf͛6'qwPKm0Ⱦ*''*iK9-qmcfRp&:'Л5%Aȟ]d6lٳfٳf͛6lٳf͛6lٳf͛6lٳdFN\SWnMXxI|}d}e̷ ue?#6lٳf͛6lٳf͛6lٳf͛6lY~u41Oؽ+ɤF F.+f[.v$$b[jq!g3FLs@G"V^i[-w7WsQ+A%B"E;l#=}kΟj#qxg_1٦YGou:ۙ-eԲ+~lٳf͛6lٳf͛6lٳf͛6lٳfȝ/f״ZNc&*I?j{L:~[+MFD͛?ٳf͛6lٳf͛6lٳf͛6lVj:d3X4s?!,k˿򏝯tm[Xu+w7q/Fk%FYW;r9<췞^V}RX#2͡4L߬alٳf͛6lٳf͛6lٳf͛6lٳf͑+K*^OhнΓr]gHtcl~yco垽j[_Ea7ylEX8%2~QH7^#j"'"lٳf͛6lٳf͛6lٳf͛6lٳf͌J:9JUj|, Z?VkIa,\jR[+VWkv8~tLٳf͛6lٳf͛6lٳf́ 2u]CgnjkXrIDygK\'?swwF1}Kڞ֮({}jD`yi.ljuw,ah.e$~(xxd)aa]v4JݏZ⻋cYk:Γ$UՀS= ((1{eX/t;i4dl~J8Vֵ}EmK  ʟSY55vi:ƝrEJ`D*#:2#ٳf͛6lٳf͛6lٳf͛6ldCoMq" 0Uijl0ju=*K"3~TWZI( ~q]zV$IilxIp)Ƕƽq]ɵ(8T|ЍEL?ލƒS7r\ySrOjwUb[M>_?Z =򾫬U܄TVܚG_ ո/ CFCfSS:Cyƾkm9jHv\5kQky'I﵋]X5NrW}25KZUZl }4ǵKԂּGi]Xڏ%=¢žlhx(mJa SOOlqԍi֟O9_rt䇏 ezP?IS{*+AN%ܽ;J֕5+SNI NA ю/^ղՂڥHP *x'x~%$ۣ &&)$6+1QņGΚ֢m7P=im<0Q#}D}Ǧ8yG,PAL~w?폪&o~,O$/T>dO? ΥV{Ө\GeUDҝ8LdZk54w{Y۽zRQ}xc+Լ^~ay8,t._7";4丸ClYbŴ)ysMkh4Nic%3ǹcwQn$Q)0ԥހ|-ƣ|fO?GZ(n׀4㇘4s)]/Z5yd#!>aїڟ*~G'kEtUDv8kAOKȷ~~:1dBC(zTxOI_,~ 6 |#lQ`rX ;h/)]Ke %zGQ293zƱJ6 zMdcZ7+B7Z4_7N~cEMbaʯ!剏ё?66tmqFHnpQqېvKyYqTszt}/G;ȑcGTw%bVy槝?K?jy>׷̰'<:g~HdwK;Ƕ/X'~ _J;~/#U=q>A.$oLZ~nnkwT9h䶸:ߥ1e@w-V7 yWё&GѰEݘsw. x$;H #hu2OHO t<˪q_MRIU*ܯ1<%ڇ[PEJz|~&ٳf͛6lٳf͛6lٳf͛[Ck 1'`'R[^PE(S+RDŽ|iRnX@C:Y['/_z@6$zxLE4)&r9~ 4P#/#BT j6!¡B *( qPʠaf͛6lٳf͛6lٳf͛6lٳf̈́PӮ.,.ۜaڀr-գ87-vH4 c<+g6F=^34j?'+2y[<4ig*IEO<=+،JB~5v rl[>?G 8R}P*07' kb=SK} `#S>޿v%p.4~,QԞ}'Z/4ows:|A#KIxGn!]Sڛ P,Џ=+MHӭy)سfrEK1;0fٳf͛6lٳf͛6lٳf͛ /N{2N%ZGdn'Հ)@+|25B5)celeE+EQ)$`h*w=ܐ2&oVpLT.jX cEWl6lٳfٳf͛6lٳf͛6lٳf͛6lF KKfѠRJrrr?ve@i$LIڀ6͟ٳf͛6lٳf͛6lٳf͛ &ZɊPoӼw*KzTqR͛6lٳgٳf͛6lٳf͛6lٳf͛jhڄhʇwvY\ <Yvg-턾j2QDYJAKjAZz DcH9^(TTrEʧyafE-ݸ#c3G=:Џaٳf͛6lٳf͛6lٳf͛!t^XZۻ//6iF*JWĭ0w3`0]]r/gwPw ӽ2o6lٳf͟ٳf͛6lٳf͛6lٳf͛|jhT"s.FN/XWON[/y{ zln$ S І$ya^]p]'IZ'㆙ٳf͛6lٳf͛6lٳf͛#~ulRUt«*+ZWqzvm狹!_VԎ5V\\rPE~Y'͛6lٳfٳf͛6lٳf͛6lٳf͛|jhT!s.F EKzkZ( "ݴ)iHeOYrs6hukRX'o{g?ٳf͛6lٳf͛6lٳf͛|@z]?: 4]D\lٳf͛6ٳf͛6lٳf͛6lٳf͛|jhT!s.FԴNbOuwj*&U6/J{!ZW90E+dcZi0OZ`_W[?Hjmm*OFZ:*ETmƣ>$x?7\3͟ٳf͛6lٳf͛6lٳf͉\uw*Ao iX""d+YnklYu+xo-/-Kir"k!y9peB떺ֱcͭ^\+VzchVgG%nq]wv! ͛6lٳgٳf͛6lٳf͛6lٳ`]CTt Ωy q"Ļ{D8gnVG@$ Pzl37&ښ?-0#Jh4wY"OR흻ܛ.-7G} w *}M^ynѢ΋4_Cj%b6U 6aߐa"4Wc}[SXn- -'Aڔ&o5A人< $:11_Ho sgٳf͛6lٳf͛6lٳf͑ +u.~;CY64 R[W +; sE%#3ɥ$f~a젩ԖG$sFDC* ͛6lٳٳf͛6lٳf͛6l ?0&77VZ-%wR-ԖWAt[Cڡ?]x5Jpphzd}hL9wpK06{gmKInԭ7WONIůZeZnBPotٞ w)l8_ o' gFꚨ$Zj!J)TPmT]S9O槜<OHNz~ o$3mqb,|iW0i4YĠi]$rȒO1j_WpZU/SF9~Q^V2ӡl1ѠmXgҤoRIky H"&Qȿ=A#$SuI1 6]ysPt;X:X1ӥNgVy[iVr$sv[*ƱT̯%jZU\;fhHfu4) "V߶Z>MWYQ2,fhՎ|0g/rjk~o5Z[O,jB>nd_>Yo͍M6P>n YxRyqn<sPj6L^+!*ۊY~Dg5矖|Xi*Ʃ(oR>*x ?1EC~5cO$5f_:yE Y1\Fz6:8ybx[yf(1gcSn{]CZ[U+I"ߊIUq8rݖz$ADvjFT"5F=)ߤAҕċ 0>C+?(4p E^!P? p/y ?K^+Cso{4$'aUW׌ #yT֭cH}(ƞh)om[M0ik[<V E{x5Lʡl37V~Xѭ+eZ?ZN cd%CF૩A PP*k!%|%"~7L"Vt6!5W}/Q5o?MFWMF1R&ݺ] o?'.7+./KH?YMpGtnFg|*m2|ȫh>#!?ao{%O:6*h2*!W~_E\:~@f͛ n-5X8h+B'*Wӌ3ȕZ{] kJ?<ߏxJSşebC&"n5>篵籷#:>Ĵ1}L=tKS__y+V݌%AY§ȾM^ o&ՂsV FiM?>@a:%W%S8$dk#tXwɿ`h朿?aH9Ε~k&j׏i4Y%X}9Tpqxa3Cq Xu2]L5 bxd׃C"?LUK>-2d4(j䂷Ԯv 0Β(b1ZZyu6Gfiz4Aqzz7$BBB-~"X=1׆V,fZu=I-+Bf̄=U Bb-"Tr(YCٳf͛6lٳf͛6lٳfdм^j]I_X,aS!wqiZrJ>%?d֚Y\HiqFݣL&hGӊn Ao&aN'Xb^zytY- kfaJۑ d6lٳf͟ٳf͛6lٳf͛6so˟"}KbY_)uB'QLrp#0~a֫ťAbVFy&<ʼS=Y3 AbG/lnnkI =( H-ߠ6|}fT򯓑VfbW7JU)o ? @d/фfϖ-nl{yTBKH6jFTu$ld7]\^W7jZiێt&t$õ](kǏ,lbmф6nKC VG4UUGU6lCSGa?/u_]al!ojKtG?@lٳf͛6lٳf͛6l Uմͯ)0E ;lDVwc M+Rcי6z0!H-- VMI"vUE */6lٳf͛6lٳٳf͛6lٰi5nⳂL=k|#:U|04V2/gUӁQ~ϾI#8cXAh(T tٳf͛696iEKmWWX{"7M,fL/כ 5 Vds͗v].#T療 -ex 6Ó02n9gٳf͛6lٳf͛6l%ּoi j: ?Zՙ @w '˳ |:T"k5omҽF|9!͛6lٳf͛6lٳٳf͛6l%|գiJ׺mQ_L'DyZ4^Z= Am /L򖉦\}|Bךjw7?d?ɏalٳf͛6lSJ:;$S_%Jg5;}>o0B'PdxVZTUp$zyʤP8׮Pi@ >Yy?BU&4@*'|CMҴ8o"eXEY^HmU0]jalٳf͛6lٳf͛6E5f]#\YcH9ZQ:Op?|+gIXhPȖ丸oRv..$=I;:(06lٰeo(y^kmd""ܿ՚8>=J5y{R#,/Z.#w@@jO' mkGK(&mvgC#1IˋF8Kq8a[k^Y[I8Ȍ1 n]p^.,Z&m$#(&SZ{⚯4=Υm`fWxVTHP93v׺եwY*ّj~c/y~_ R;[s$ $qϛBe/7\!R ^y~ZPU z+} OàKs+\m(j cPU &h?KU$=2~zƵ)Mӵ-^4hmd k.~,k77wPZc584e %)k]N[ =*+;q*'3f͛6lٳf3 YqiL Y/Md` GO;ꩤy~))yXy]TT2qNOҺPzayC9GUZƩowzL64cOvs/]^l gU_SRX>cᵷjU#~*A$rp_.-&k ַOc8co#GrLٳٳf͛6lٳf͈^iֲ_̖)yEQܓ<#_"mFlevv}VV0 "P:Qf͛6l~tE<ߖ6<9#RAs E $~dy?Nק|;̐X/TШ.[zdW&zPJ$"6lSE mI躅k{li$ )Nk[ȭy9`=SlԻ5.ǾyL<_[u{bO*@n U5Ԇ ynm[CKkK̰|dRKHjǵYՇ%C5_%M I),óҌ#컰a:6ٳ͛66%4ϴ+/:ך(1%յʡٳ͛6ٳf͛6ٳ͛$č$ɳI9źL#^%ٸt17O%ϳ1c,ȵ"FB* uΫ0iVKIn^K\xf~ޘjj{̗m "Q9gٳf/gcsw]IO*[C)aWм{-yDMW{Bs3ƨWhʼvMM;KXQnfKhV i+5'b'_V.t}BQy_SԔ~^ɺtV^vbkRFMOFǦG[_%[dVi`?n4B[,ncOB#`)F7F;z͕m>k]Z:TZq#jF?TҴ։6=ɯAi~YAk)0>6U$Ӯ 5@h5N~ ԭ=?uۍkW4u[;z3ڗkhq֭,Xf#2X&׭u[y4s8 T2VO:t9WXԟH{kf^|-NI6/;J}d~bP?+X}du_Vոv5;⳧N#ę998'f͛6ٳٳf͛6ٳڭ7=2=M9Ӱ܋7±1<Eifɾc|t~IҦn5n55RI KH;e(XAh(͛6lٳf͛#>Pǘ!WCwhX*cQo*.怏Q?-hnm&F? sgٳf:}孫Mm$vR܏l>ޱӯ漊obFq38Vѽ:´Ǵ4' 0{]YyO^xZ\k tKdKDtG Tj~cXӵ)%59oFdHI[PS=Z6浽2^(qUYUs.K9^>x`+vۗhڱ-U36m4,:D_ڷx`gƟwuKelm}hdoI5ྒ9xSDV?--.4|-RI#\{p"y+*WhTi-?M b<$`fxe<އ:lٳf͛6lٳf͛6lMyA>=ؚhstI Lڗ5u+q7K]ejJLrv׀?uzW-YY[-R8b@U@͛6lٳf͛6l8c:׷$Y=`Wʺt-Z,nOd6ٳf͛6lG7Ik^ɦ9~ @ ٳf͛6lٳf͛6lٳdCN򮩫ǣK7+$t"#==+:\Ƴ8*R |S؋kEY'ِ#S^Oo2~SWՙŸt$2HHh:Lu3jfO}ZnX#$>V>qoƆ"bH8X/ Tw8} }wJУMNDf%`UVb%yTW3h'=/uvdԘ1:?=^[Yړail޳i&an}[JƱ|[>f͛6lٳf͛6lٳdw`tGV)6E#ItI#]R!͟ٳf͛6lٳf͛6lٳf͛6lٳf́u4M6;Zaa^e_8.5ǒ+,l7鯣4JG)k1η2:C֞R =] nAeJҝzd7ͪ'?0_ҥcqXCx >plvA֚ߔ䘯{JR;ŏJxsNLI*Z!c93(Ç{o:rXyZ4Weiɖ(5T͋Vu+'JZPv;6Q;$HuW 4O_0Ot,;Y{[-NU6G L 0(H6~lzicmq"Bn}(0fh6lٳf͛6lٳf͛6lL<+ׄzUBhr' ).! CNJrrq>PP]QASV{78}?ٳf͛6lٳf͛6lٳf͛6lٳf͛ ?U\m,^#mnuy?CyZnQR$_Y^w>CЭ4Z:uYMd.R. T~5VNőJ[Ex؀*R() ?Tm+XGYR9dQ"nF2]Hk$]L!ymXdb*.lmYa?D`J|#oa򇕯51sj!#HY~F)8aC6)\qxDJqWD Wf͛6lٳf͛6lٳf͛/7h}bi5TҳOO\{=1O6>Qc-L]>?Ec4A Q`qMy;n=Xx~ٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳe;hHff4 $6wZw}WvqJM| q֗wwpsxYlEI&(q 1 j+.Pcyn$eU>lP{,ռ=4.TJ 8Fjąik^'e{Zͪ-]'Hg"?{K}z`ai5ҭ"S_J =YՏrw¯">R}/]$9=_ːN{ˊ vϒٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٲ1h4.ZM%bTR~U;W.5M3TKuBP|&?;h~VѵMKTK5frEd]4&Օqri-L}+r>{G6tm +*)uNO$^Z2h[~y/Ϻt7:_]K ROl"ܐߧߙ'ϟIO^][rzjD :9un|c]Ǘ4}V1 Ncrx; v7Ϫ-'Rݼ/,贩/VXE%7xWӝIo|u+vN3k !wFC~iq.kr巗|mG.V}K%̨.>)<7Cy2iǥƀFihGc50k['p Oˊ^I9JM^;=3Vim #Y}Y =;֙xu 6nsk@j<+d{jbѦ?jpe[FHq;[#H%&۽H%5ļ]'gٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳ~db̺nI/nm\G%^+W `-/u 0j:Ĉ>R8  u?$h.-Λ{ KU2?<4Isj}>%֡5բ#/M;Mu=BZ'm9no^q[=y$?WH_#~YWu櫩jh_I$=p&4>_0r5 KvoV9lǐRkڔk&[swˤ[2I@Kִ½[H5BCQT(ڵn>"f ha:y~MJӠ-]=[No56aw={FRJ|:^]y[~c,.=+{ T vOmL-Zb㗕c-6F»RF[xiWNK62?}Pw΁ikj4Q@ 1ٲ31k>jX)s?ﻫKy~TIl!o=\m4 ;$Q#P< ~lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳdoǬi]>c c$WT۩C,UO/Ӓ<ٳٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳdkjv~cKI\쪻Z2uFH%E6j";ؿ8__i2 K?D>|Ifٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳe2G Tz8t/5h6Ew[9/#K8uV 2?VMCh,di[Gb|N͛?ٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lD@P*I0 rZ@7UdQg?VH$7(HMyvB8:84: #N]|5!P5!E&Mؘ_ԙ |FRZ3/5mJ7!4dPpkX)OM\ЏQ65e_捇|3 KNoy'r;2(GW],/?[ϗǟ*oҺ]xv޾W/0 ][_0j؛y\yӂNch~/Lѵh/E0hՔ G6l]B 4cK۠olX $T:8\5$b~^6zF_lν~+i{mvvUߜׂR5jW9f͛6lٳٳf͛6lٳf͛6lٳf͛6lٳf͛6lWZ5\xJ澡PJx4ZҐF(UۏTS x}Ҝ|<6xܓNaon:Dv$}_U=ҜW)3kf4(AUOgf"0GB{oS TЏpZ`n5nS~E\nzo W|+&g%)k9Bn/4˫{Ud%᳼LS4kΕ }7QŽ̪f6x4igXSbҧ>?׼'土滸+ӢhŬiS8TPۯZɽֵ=[ʇY4 ]9'H:Q9>u4;wR4բխ۳(ox%k:\{W}_A }k_e>^ϋd7:XjYIG%k0+vdZ7w3j/-վ Kjy?P(:flٳf͟ endstream endobj 12 0 obj<>stream AdobedC         #"""#''''''''''  " s!1AQa"q2B#R3b$r%C4Scs5D'6Tdt& EFVU(eufv7GWgw8HXhx)9IYiy*:JZjz!?ٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛w i<\2HD}5jMMiy8#?FXtN\$O8Ӛi/<ӯHȭӫNrW&GL}LL}i#3$N4c&Q"4Yj mcy wK!M;dPO%k/gux$կnZyUs"uژV:U柣 sSOoג'+@?<|sX#.46N'3It>𴡹,I|"47Z7ˈ.ٞb;4#۳"1Ӷ7nںO(/@V;8܍o%HJ$)$ƬݪvbⱹHDߘձOnĉϱ;/~]C5ڰ]0ݴ6Wڴǰ5‰l6[ݵ,<=kc.J8Q/|iՊH AȒr ;^ռk;ek\ڙ"n+#IGO3f͛6lٳf͛6lٳf͛6lٳf͛6ٳf͛6lٳf͛ ۽ vG 8+0z=!#jt{cqĵ*0-i}3qˆ+,oƿN55̔b2LG*GJ~/SWSP6 MGYÜuF5&@ʃS)yWoO ~r\keXϮWeլNazRzlsqwz?˳D\ s,@M- |ۏ41 5Բ@h+$r)@9+?/%մO̾a}m4a[}U-;՝@}p2~Uy9|TԾqI^/$s^qe6x#¯22!TTdz[TAOAt}-rhGԛU!_TK23ʲOkoP A_a"W#o*=;y^ "=0ȲzqӐw3yK~ZQgW-y̏oV2 2?8M\kWq}QIp z^Ž~,#ֿ7t-"i(ⰵyRf2]9m~hS-^[$/#(K!yy,^"lxc D9*9Dߩާ:lٳf͛6lٳf͛6lٳf͛6lٳfٳf͛6lٳf͛6lٳf͛6lٳf͛6lՕ~a~QyaֆC3ȯEt:}0ߧյ?<]Z1HdYqtgS4&hhB,P,NREMalS曫,Wb +QyigGoupZCq8MFUNrPRWt?*t)s^>`ha3? "VA/N1:omVuV@R~xA6GHW/ӏ]jr?HIO՛TBOTBOTBOTBOTBOTBOTBOTBOTBOTBOTBOTBOTBO*ԧ=gB\pCW$u'P;UrҔ!?\&`Դ.5&XGצi¾H ߔ +.iNjɮuh]PGtٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛(w{P`o˿@l_4%k٣43v^<}5)6|EhIǙeq{.$@O3/Jp=d5V ٳf͛6lٳf͛6l؅嶝gqx+kXi=#R@ ^fӍϝ`WQ@otp}r 8 o*,EbF%E6#) ;?ٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛<&/{˼P?-:M.IsdoBqz+~ӇWt]F?Թ 2 f͛6lٳf͛6lٳfD[v?ӭ{xS1~#F20*zv )-G V!)>Taٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛3\M7]gZɥ.l>uTˍX. 9$͛6lٳf͛6lٳfͅ>f5= ́yf-w[9=XָKsˠ6'nlu>2VcQ^"t{ ),mV!sԮ|k_kYF~¹IWgmZj6װsaFA"r͛8}_]a4dBy}vH_ӈM-yuhV.-F_y!w%9WOz)h> {Q71l4i˧,;˂ՒF+ϳ71$7̚ޮ]5;ϼqbӵE.`^FOQńc½COPm3MYUQ[Kx#eG9vڟ} ?:ƞe$Vϙ qG8V#uٳf͛6lٳf͛#{*:EܰeWª?%!< FMԗ RPv%@뷀-Ÿu}emr.y, ŖCs)~妙a=%\*;C%_Qi~ZWTvmF.eԚ\zM=?Sp_q<^\4HKj.äW^I^NW?/[}XZE{濺WSсˇ(ntu9f+iLUĮ͛6lٳfٳf͛#~̿˽0;{ǒ ?5-5ƻno^%7b:7P+}60֗5t7Ri8]+OtKkHz$o9ٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛#~̿˽'ZUCGdI'u^i^-+yW@7/N7Xːȭk?ԗ兖i죐rG^Mj:O9yR%Ţ2(8|) r_pˇ@?r8 IDQ ;0b)džco(ˢnvDD_YԠjzR@iyO-a ,dS[.NM3Xs\ia Oяҍ<°?Im/)oXEem&F‡B5jp~O˖:ZM-E̶0F֌/ukg-tI4 j䁒 #ɏɽ<ߚZ_Λ`e,/Hrż=i2hvVI޹&g+`Yd~:W=-6lٳa# -ĢQGr u mbt5ZƱөĜo&;yBTLٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛#~̿˽OB<l$GTWSXT})ژSӠt.r 8IPITlGBѭ|hl[İE$ NeUE~CkIe]BmJS9V+,C*pTbAZq f͛6lٳfd>ҭi'-훲϶i+_;*N<ٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛#~̿˽'ZUCGf͛6lٳf͛6lٳf͑nQ.g[Ewlm'E9Z*T;_#Z($qJ6 PpG`vɥŽ+qk*O ч-A3ٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛#̴qwaD#_ʱh6lٳf͛6lٳf͛6l WQMH\*-ƌj+I Ru3s{Ǜ山{rrE!oJDZ (3Wyo<BHIjWq4 $1 PjOp~m#̺۽O(44Cq!c(դ;"0|.I a`Q̱JδZ8M^}cae|&JH dS]/5|]]_H$6-uՂ+$eB4I⩥% y#IuBpqlٳf͛?ٳf͛#_oȞdo6v*&_|?&y2ialٳf͛6lٳf͛6lٳd̞Y<QٕW+昱rWz[?X'yFE5I#"Rڂt>TӼP47w X9vX!ŠiK3ٳf͛kw6 >FGSAau5dJȯ}iS)_N1~1_~6lٳfgqɾrּkW.m;yแJ;xnU|fݵ]^=bG%,d0~"Ojs>o6}{ hDl渞ugj܏]I[71h?O 6}άH-S¸|71}g_})}H}8eb'|ZYYkm6QM>Xn ER$v6muk}GtTV?/e5 zo[MףF8)lIoMqlgO5KIHd5>a.UniXSo0YWC[[%V8CvE84C^>^9XEuQ3Urw͛66X6dY#aFGps֖V֗ɨ &M&1bz->3{OZiFT'+syrN-m>^Ru `旀eN宩=iaH֚tahDh訡N =?ѿk"cs~m"cs~m"cq(@ У1ӊi3I#DpVC(sH`C#!睆ʈ>ԇK/-Zi[,.Z)t렜rC TqʥI޻VҵjyYAJ&44Pq||os[ J),'xƶyo3WmX=+́v l*Xhi:ЍOc[KX4! ~'@sf]6{M͓Zq[y2+"Lœ+Zf{04D )*,s¿K6lٳf͛6lٳf͛6lٳf͛#y|(l?0u9GGE[hi23o'td9$򾍧ݍ_:}CF:t,Lm嫥򭦖0jwO*O \/"X{ nLƽcw x}ma{~1J H]VזM"u;#էL*I{VXAc4֕Z姙l-ʹzVjOcw%݉R5Jƻr^GI|ŧk~VqyF}x\E%[T8FϣMkLnlwp9x;#3Ec`#t9UHӤ q<֥¹ޘQ/~sD@ K$1^J<,* ݀L&ҵ9l? .k%m4aPԯa2VYӿ7{)H>>tV+P{VQ ]U-$e <ޙ/ iiInhu[H-q*֛+93[][^@Vs%żsDч+6lTִ0j+`l洷nn夈Zqe'Wc-m|ځ^Sދrjm-n2zo"eb=y+=?w%ٳf͛6lٰSidzT1KHŸSZdZV.?:WXz:"* `g^@Àry3Gq w8UxwV6lٳf͛6lٳf͛6lٳf͛" `}Fz zL9͑)^4\H]}9tOTrXw[nE觧 P()ӵ2--nRU!eEpt 0;渴ѻ9ʊQK2X>$( PvE)-m‰n ) +x1&6dؠ%XEN>PghUBт(mlgi'/Rߘ MUwPj: X(7>Ҟɽӳ=󞰿ܧ04Ѡzؗ<ߤVփgK[?ʂg0|Jʞ˃43ڜRWӮѭf?PWl Eל %Nw$j>6>+mZZYi;$vYڍCGV1l**iXy OceohFmn45 DrCٳf͛6lT,k)/Pwv6tp%B(v$?Qi=.{=F*-@J|.BA[oڡj?NAֻM~%FXKhVw{kak#D?~Y"I&M/k/W@Ħ.pp?ٳf͛6ljZ5zp@ݏ4Zܜ'N?1y=9ҴC%0B*0½٤W8mF&^[*:D,L~0;[]j/s΀/L2͛6lٳf͛6lٳf͛6ٳf͛"_?/0o0_im& 3f͛6lٳf͛6lٳf͛ m6S0><yzMNMZ񒏤s!A[W*Yz ml9Ck1oS*Ui@) ٳf͛6u=&[DVɷ$!UEK31 7'af{_E/0Daxjt)"Xq3MJu4=|+LdRK($X_]Te VG$3e!vw/ [ϣ"gP8ĬQy1; 6lٳf͛6lٳf͛6lٳf͛"_?/0o0_im& 3f͛6lٳf͛6lٳf͛d t |M4k^[9mt YԮa$_*V0RgIgDĉ@:5N2+m:%ƲzBТU;lJRbVTRGޓkqjvڜ_'I‚;Ӷ&۴CS9Z"xVCUVI]v)vCI˨Jj"ҠM6?B?0adY_5 EXUU\3$Z' KNQ7m5IM6ZL &V%l3@(a6lٳf͛6lٳf͛6lٳgٳf͛"_?/0o0_im& 3f͛6G||Ū\V h7BIKQxd&coP5;WLƐc[c m $L9}M fsZpLԦc7 Đ DZ|7tX<_%%h &%=j70q}q e499`^@mq Ɗգ1huM2' %KZeTaI ߛmtH4+MRA1&,Sp?kͬq[B8A$ѩWaQ0֡a`P$m4fRTnõ"vC+~7hø‹7hv2X3_xcs#ٕ` "qR`=.5'Ep[RՉP3Z{OL:J_򭇙?x%HÐ2Eu9)V?Sn'KmF$)bl $ ŔW.n忺/`ktjԫnn#jwྣ:KބlCzSUG*}KPbWI}:DfhUCFlߖߛZklcIte18%܈尩ο6lٳf͛6lٳf͛6lٳf͛6lٳٳf͛"_?/0o0_im& 3f͛6q,Eod1[(eC &aK1~`O˾k& :vFqyܩ!vԋG\֬bdx%^I4Ϋ35YEO/.^\.QZY]d@nťz2sÒ4C^%tݮ"{ceO*zv?K|RXoo #OJjSǛR1_o7/oNXoi_u;k]K:4ɵMf [t6yW+7W:(Z(Fu>%$*~ҕ5ޞjO(w˶=Fqmw -%-ahO݇zvxԧom-IzN3k?ZΖ]?m֝>\ʂ^JܷNƴ}5G(Wq͛6lٳfٳf͛"_?/0o0_im& 3f͛6lNx#[i(FE,*#c!w厏a}ay61r+-Xɍ2k6y-l'##*x[l}o~4h+u\zђ[WRc۾4+iiijwIfs$!36͛6ltѠ=]Ck,3*ȍ,2=AɰF/uf&uhnV}NK%9[۸EQEt!oRךfQj֡hQXSi|t;vRKk946iųviXeeط_u*LѺ1q&mH[E""ܽ:׿\$͖Q4ح-}r=^;4(ޒ( ( rghڥVh.ʺ|X}>959-WO(Dj~;a/iכa/iכa/iכa/iכa/iכa/iכa/iכa/iכa/iכa/iכa/iכa/iכa/iכa/iכa/iכaLk0_A59y@mN}r ƞNoFS(kҟƟ<( 2i@xzeKPlӭoߒ?eҿ:b֞os]]JipI#VöFS1]+1E]2_ ǾG]+>1FkK(?#ԵFW-iї7KZ_!A4f/3|R֗HP>FkK(?#ԵFoZ 7-iћoBh7KZ_!A4f/3|R֗HP>FkK(?#ԵFoZ 7-iћoBh7KZ_!A4f/3|R֗HP>k(?#ԵF鰿֗Nт-Lk r!"GDm8? -H<m&bEV/E1PG3*RdV W%8 /i;Cy~N{L;Cy~N{L;Cy~N{L;Cy~N{L;Cy~N{L;Cy~N{L;Cy~N{L;Cy~N{L;Cy~N{L;Cy~N{L;Cy~N{L;Cy~N{L;Cy~N{L; KnXwEii߷{wYݧ|skXVA4XDQߤ(0?_= A9<͛6lٳf͛6lٳf͛6lٳf͛6laQ,{,sB-q|/yMȆN~sٳf͛6lٳf͛6lٳf͛6lٳf͛#y~ZsH+o4iמ[sϟܓ sf͛6lٳf͛6lٳf͛6lٳf͛$40Hwc@$/0ĺR?4BXYV߯NTbs^_.??/iWS0ްT>5ٳf͛6lB[8'{x^N"5jwa{dnΞ.b7Z_TGZs1חf͛6lPG+vGE,bїf͛6lٳdcP/1> p_im& 3џ~\wtsrtk6lٳf͛6lٳf͛6lٳf͛6lٳR6?-)Y_W,IOVaSQ1wҼn6VmӲCl9 $zǟ_OksvN&cӲ՗⯁o-[F=1A3acJq+S)3KsUTc-IVS70Է?%ZLߦ<RkU3~KsUTc-IVSX:F 3Hs)uH|)tkx{lxD'\+5?/[|ɧm}V{gk1Y!%PF#K>wԴK/d|J j)![Zp8*[ky$;moZ{!x²ԞH P6ƹ3f͛6lٲ1(q8gG4IӞ\5? 9f͛6lٳf͛6lٳf͛6lٳ`{6-46r@ny-E'Ӹ=`+4;2OO*iP^w7pyOs;nu&#-kܼ̯_!/c!j%;ڝ銪?m /"LKDr /-+6lٳa>+V4v\Ver l.qI2_]2]D gm^i} tbEӭn$fAm((|1x"[ sw3Cm"(fI':,7] VRTuzC}6L7o&‹cuqGN t⑪D~4ߗDԼO人Z*#wTi0eZۯ`QTSԞ+?ٳf͛6lٳf͛6lٳf͛6lٳf͛"SDwo7G[ɤL?j͛6lٳf͛6lٳf͛6lLT v9~kk_r>Fh-5+qP "2Gt&BPX3qG E5QU 1ٳgٳMܓІVVq6lٳf͛6lٳf͛6lٳf͛"_bß-9L4_ ܩ.F"8lٳf͛6lٳf͛6lٳf͐_BK'ir?4zh&-Yi_b miZK1 &Y1hm%Cj^p=$߰tlٳf͟ٳ">R>_q>i8xܶ݇Kf͛6lٳf͛6lٳf͛6lٳdKG% yn">m޿Ӟ3͛6ٳ͛6ٳ͛6?ڍPⲵ5ϳkհbʰ)ܱ<$?֍riegoYZ 4EH"/6lٳٳdGyBD--s YKҫ xuO+yzL:bvU6m,Sqf͛6lٳf͛6lٳf͛6lٳdKG% yyJhRBv Dc_Q0ܟqf͛6lٳf͛6lٳf͛6l؅ญ%6#_ճ(۰šK㶶VI`ݚ! w| 5{HJ̪ghd`%͛]Ky]Kש8`zlVIbyt ٳdOVm=n{F-Lf͛6lٳf͛6l][\j:^6 #[E!ޡj%7j6Vڅr'RE8#6lٳf͛6lٳf͛6l{2AOh?j͛6lٳf͛6l_Rą䔲i W;:e]ǫ㸾{KHmm .2(PH'}MUO0EYM#GUoL#`z.Z][we2\[L&qlٲw$6؍Mؗ~<͛6lٳf͛6l#AִAz"| VYmźayf͛6lٳf͛6lٳf͛!$˯0,} .}?ak?j͛6lٳf͛6li6vWX--c9d9l+ԓX0iŶpSygivrGn/k]zuZNRI&#ibHЖQE#l$BK[ [7ܟ_M؂TM0Vlٳau-JO[P-nf,*)Uv^@T#57v^D-' b6IЬ~mMMy3\4z&pn l,Kxś]{O"Wڸ'%n\B؉)OT[$ ՠܚ4a)1jV}nK#GO-iꎰ Yz[f-ڊF`:sYР+mm;7.4)m$D)¸oٳf͛6l()opeuag8s6lٳf͛6lٳf͛6l~mïLi㍅ִ1S?j͛6lٳf͛6|ڮy۔GDpLrq߃ũ6ֵnFjvOBn+5'n'nXM=4skrזbҝh.k{li[ܴ,n@FY6?T#7 ɬjP\YHtB\YZmys*Acg8na+qK?:s2<ȊHÜٳf͛6lٳbVz_um&C25pF,AMTt6!}dk:|ʰ>6"~ɻVI ۚ§p|+0d` 5ٳf̈́g4҃vKoVc LU4? f͛6lٳf͛6lٳf͐ͯ>`u1G#?_"S'f͛6lٳf͛6mJ7OY#PEp>QK2E l¨,V9UDWԧVoicVRSou ōҀZꬿ|ffZ/_9m+_JuX0tnA<ٳf͛6lٳf"AȼY|3[C^Ɲ#KE2Qq6lٰOiewufת6K >0վvճ۳f͛6ٳ͛6ٳgK0:]mџʳ7+<ܱ3f͛6lٳf͛6lٳdTAvZc];TC9%>|$a ٳf͛6lٳf#IQCૣ PPr;mc>zD:sKKٴi1J?Xp6lٰVZ+ԤwzKƹ zQIobD*yf͛6lٳf͛6lٳfWq nF;QFӞXx&tٳf͛6lٳf͛6lPnt-ouC($Gcvu]SN_NNGYi7͛6lٳf͛6ٰ6cg599$RetuYXڵݍZ %,5 zVm>TT)lٰMquk-6'i$œpoxR(/ڋͨ^J( wRE_f͛6lٳf͛6lٳf͐o?c"ɍm՟X>wzl|~,Gӓٳf͛6lٳf͛6lٲ?uf8( R]gUZŶꅆE,Sē$R(xBYXT2cf͛6lٳfٳ`k>T!5䆣u<V05 ,fk$ZN*bޞWe&mz6$0 Fl,5SoPoCN >e?iٳf͛6lٳf͛6lٳ`-_R]*K@R+{u 4J(b{u-:]3LK/=^/q;elBL1=57̵{z`۪QKmto,nf_~x܃I-Fڿq.TfMt"XYdBHF[vqgGsjWi_'kRQ,m␀G4X 팏yyVeҟ--Q2>ukkkeZcpŠU@͛6lٳf͛6lٳf͛6lٳf͐o?c"ɼa~CcKڿkE_'ٳf͛6lٳf͛6lٱ =:ݮK{tie`V4Qrv7-\q/?a#om7Tom=x Z1K&EI?Vv 8Wn!UUkF NI7ZU⥪Y4Z =zLЮٞ㳆,+5 i[Lײ,=^ߌqFe6:L򗟼4o3_?QӚ@bMF!?br4cwY-PgYc-. sAq[])+H_G7O/#-?uTs=Q+H_G7O/#-?uTs=Q+H_G7O/#-?uTs=Q+H_G7O/#b~eWߔ< ]HQ( PS ^k+c'uK[켡EpWBPҡQqEs4mmmcCؙ\[N({%8D5;?_?u_4~vY]ӭ߅_,f?ߖ}k؛[ɜ~]~kE޻iնpơ񂾘q j>EHcpY^;c(;-]s9[ѺK#2C!Ix~Y YImn>N;Ww+eKВ?wM?KZ!>̫HXQ9oVsOpW^" ,feWHC/Wo9~jt._+ϯ馧] ,feWHC0=5?iwT3̶?._@NܫJ}R7O"]g1Ca~yQzu~[IހПuCԚ aP?:֠Sud+&2O庂[VpRl:WvDc/{Y^ ('C/W'\+W'\+W'\+W'\+W'\+W'\+W'\+W'\+W'\+_?[Nחngյm.P*X§Ȣ ٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6l endstream endobj 13 0 obj<>/Font<>/ProcSet[/PDF/Text/ImageB]/ExtGState<>>>/Type/Page>> endobj 16 0 obj<> endobj 17 0 obj<> endobj 18 0 obj<>/Height 708/Type/XObject>>stream W 5< =z].K,8"#$>~'_ /޷ia% /_u خ\\Km.]YUi6M鶗M]ڝJ0-}{iӽvDاޛtja;_:"_V{A}So 4I7]P=M&##=ꖖkԵkM'W&L=%a_~\A;]2pO~pbZwqJ/8#8o/ A5?! yA܃AÙePA'ǃNV%yxD*>k>|AZ¡!## #:(#!"$0\aL B : S 駄ONk|D=Q;PN4N : ֛TjN_]iFWWQ&Ti?ms K#}tJ-,5JzI.//܎#%T-wM_2?$p\Z #AzK2W+Iw*]kCG.=bE`>/Font<>/ProcSet[/PDF/Text/ImageB]/ExtGState<>>>/Type/Page>> endobj 22 0 obj<>/Height 735/Type/XObject>>stream LrK s2m2r(Afy8ҷ%tO4Am; OWN/5Hy!W~^֯;͑__U_E :^H捴6պK$|H?пϱ.d{\|T֓kiK"7]mm5a`z aM}}۞__ *tv}l/b++v~vNL / ! uR'?ouTӵA]7O_KmIpˊw5y8˺v8N0qsYgv2W@+Mpbx_ߏc*=%IKYDgd4p9 ttg͗#dNF">9  ayjzi ZEqq/KJ$=r1Ȯ_},x̝M?W I+ q揤nAIV\=_: X?|FB??[5B#&#xoڞn%^q&>=:<mmH׿awﵵ%aaA%ǿT\T{"6E"iGz0 8aaU?,@ `[ά2p?;ASe.?TtuEBIilV/< I~i%`43g8$_II$+2I$?ҙI$_$OFޗI Du_$KjZI$Q_I%bI$i_I/"#AI$K$KI$I/IG\I$I$o43LI$$_I$$KI$$_I$I$!97=A[2$_I|DDzI$I$I$RI%I$DuI$I$I%I%I/I/I$I$$KI%I$$KI/I$$_$_I$I$I$I$I$$I$I$I$I$I$"?$KI/I$$_I/I$$_II$I$I$I$I$I$$I$I$I$I$I%I%I$$_II$I$I~I%)$$K$K$_#@I$I%I$I$9oI$tI/I$I$I~I DI~I%I/I$I%I$I$I$I$$_I%I/ +I$RI%IG]$KI$I%$_I$I/I$I/I$I%I$I$I$I$$_I%I/I$I$IxK$I$I}$KI$I$"?;)3\jI$$\ `I$$\&i$_I{M4I/I$'N(8$_Iznۦ$_I$I$I$WW<2I$#I%iI%I,׿V<͟I$I$%I~I%I$_I$$_II/I$I$I/I~I%I$I$I$+s5$^I%I._$]tI/I^I%I/I8I$$AI$W[I$$K$XquI$$_HqQQI%I$$]$KI/I$ i)$_I$$Ka/I$I$$\$^)$$KI$#I%I/I%I/Igetc>dxI%I$#I%%a%I$$_I$ξdI%I$I$I$I$$@I/I$I$I~I%I$I$I$I$d$}~I%I/I$!$dAA'>I%$_I$ OKI.I$I$?UM?$_I$I$7mҢN7$KIG]$J ޡa=?I$I$I$}oI$$_HtqWkI$$_IkZ`2I/I$I$zm%.#I%I/I$lTCKI$I$I$GI$I$I$o $I$I$I$c.$_I$I$T8AI?I~I 뤒I{4I$$_I$wI$I$I$_$_I~I Dm'KI~I%I$ڜyI~I%I$W^I%I/I$jcI$I$$^_}$KI$I%hFa0$]tI/IyR$_I$I$kI/I$tI/$KI/I~$KI/I$I$$KI/I~I%I$I$I$I$I/I~I%FIdI$RI%I$$H":$_I$9ܨ*9ZC4sV ,I%I/IeI/I$tI.I$$K$Z'#I$$K$]~I%I$$GI$$_II/I$I$I$I%I$o$^)$$KI$I/I{$@I%I/_I}$K$^I$$AI$I/I$I%$_I~I DI$$_IrI/I$I$蔄RI$I%I$L_!$$_I$$K W$^)$$K I 뤒I}$K?$_I$I$'$_H":$^ATI/I$I%I~I%I/I$I$$@I/I$I$I$$_I.I%I/I$I$RI%I$FƿI$$K$_ ]$]tI/I}% (_$K$^)$$K$_H":$^)$I~I%I/I$I$$KI#$K$KI/I$I%I%I$$_I$GI$I$I$$KI$I%I$$]~I%I/I$I$I 뤒I}$KI/I%I/I~I%c$_H":$_I$I$$_I$I$I$$K$^I%$_I~I DIwI$$_I~I D~$_I~I%I%I%I/I$I$I$RI%I$$]~I%$_I$$K$]tI/I~I%c#I}$K$_Ix!I$I$DuI$RI%I$I$I$I$_I~I DIoI$$_I}$KoI/I$I$I$"?I$I$$_ItAk$_I$$KI/$K$_I~I%$AI$I$$_"c$ P?I$I%I/I$-ZaI$$AI$I$M4$KI/IxKɾMODI$$_I$I-&m 7I$$_H$]==uI%I$$_H$_I~I%I%I$I$I$$Kc_I/I~I%I$^I%$_I$$KI EI$I$$_/$K$^)$$KI~I 뤒IxKI~I%I/I$ I$I$$@I$ .I$$KI/Ie.AIy0$KI/I$I$I%I/I$I$&I%I/I$I$kjI$I$$_I8zHҾI$tI/I~I%?I$I%I/I$)T3X9ᙑm-~I%I$#I%I/=A\kk ~I%I$I$RI%m',w2A ~I%I$I$$K7wH8֗ I/I$I$"?I$6T`I$$KI/I$^K`)$$KI/I/K%KjI/I~I%I$5I/I~I%I$\4aޗI/I$I$$_VTRZI.I$I$I$z޸-I$I$RI%I$罹*sI~I 뤒IxK8i"q$_I}$KI$Oi'Y+I$$_H$_4KcI%I$$_I$8$}$KI/I$I$_`)$_I$$KI/zK?I$I%I$$^ڜy={I$I$$_I{mlzQI 뤒I}$KI/_I$I%I/I$c࿤I~I 뤒I}$K h4iZI~I%I/IyD$_I}$KI$$KI/I$I/I$$KI/I$ I/I$I$I$?I$I$$_II$I$I$$K OIzI$I$I$ hHuI$I%I$OI/IxKI,iI%I$#I%I/#wI$$_I$I-$KI/I$I.I/I$I$I$$_I~I%I DI/I~I%I$I}$KI/I$I$I$$_II$DuI$I%I$$K$^)$$Kdq?I$$AI$I$$KI/IxKI$$_I$I-I$I$$@I$ I/I$I$$@~)$$KI/I/I/I~I%I$I$I$I$$K$KI$I%I$.I$"뤒I}$KI.I}$K$_II/I$tI/II/I$I%$Rh $KI/I$I,LI$$KI/I~_$KI/I$I$?I$I$I$$Ka$K$_I~I%I%I/I$I$I$I$I$I$ bZ$K$^)$$KI$$I$I$$_I}$K$_I/I$I%$_%`I$$_H$_X2I/I$I$$@3P}{KDAes&m̊̅ȡuF?C/H/]j#J>Ҽz+|HX3d$2 /'.& 62칦] _iZj?ׯ$)VҴZWXi/Kj D)l YJ̖١VJ,53Q!b330fS g06X `-ç XN:m֚qui'yܝA7 ,u'd/]=M+]iQWOC_ɢC??Ј\k_m'O{k__5LWWdA{M{"LtM;MtӻAwȴ0 endstream endobj 23 0 obj<>/Height 1550/Type/XObject>>stream Ecu}~ȡu}~~ZʌgCA~Ӣp8p?]o[[_k;iL ؈!ᐍPM|0|2:v P+ 2ڡ4c*G[ R9Z߂;Oegig"Gڨ,ّh̆# ,\y|\\ ,PsVGE-> 'ph9tܤ 9n|X)ɶqɱ7\ ɰ^Gɱ)o<.o ѓqT0u r)|-ɹ.dȜ5Җ@yr42,hiiܲYd4Od-uYlY~ nSip/!i4( . v `f~YAំA82d. A@#1 aux70̴g%떒lY2w-? d<"-0 `eaJ d2!A7N7ۆz}}z(5yJIk~CMW07:}w u?>|0;) 4͏7Ñֻ\>׷¥u޾¨`c;ᇝ{yc }L NZ/?ț(hCPA}t)wYz[$Z`qWג?j׿~q9?VVLKMwxka b" Z]>u?įa].!ӯX\1N"c.?T!~?&C-SZYx^dMHu }SDTM. i&?뇺B:`:U7[:Җ[50j}pa`!k#]L `i4wTk޿Y T~]n"baZVدp hejNOv8CcV!Z ]=Tj-VGDnv83OO\xhſxeP.)D_;G EO2d9q~mH&ydѸ0qگu+a[a-e9̎&ڳl }M@/Rn@pDɻz'ztvO]ĖZzἋPޮXns_oO>鴬=;{M5oa4Շ0"6o|5|7oo oa?i?yi5_ֿ_Qz_}KIK܇kOi_wU1ZV8ZjJ 7b߮0a`;-/|=aC v^:Y}w9i |\"99w[ - a'oXB5..9zq=?vD_}[O]EG鯆a|Dw{HU??nY?aȣ!A04TJ'nZzkWz]!T(OW~i*?ob5 5!-"C߬4_-%׿ZFhw>ZposAt!0ꮿOo!>P$nz} bw;CZ~km&q_i;L( aqi~{k~ޚk_DZ?[֟nF"]6H2[Kֈ-X C82 Ür73K0CX{(rAVU{(Ü2MPuɝu]D^t+]ګ^q:Hα\3+]C+( th54M{N498 wDRzup IG}~Yr_D4]iwGm-z K?֗[M!}'[iZB $Aukcc+4׵iZh0"1YCgp3[]X3_tEa>/Font<>/ProcSet[/PDF/Text/ImageB]/ExtGState<>>>/Type/Page>> endobj 27 0 obj<>/Height 1504/Type/XObject>>stream b~K޾|]vZC(f/AuOOhyz'z?'-  1g/^ vom|qQ]~Mv GDO`i>nxMb:6{#k}YU0z )y6RpB_6(Bz6# . u'gSiYjHt6pm+YuZ  ~5.(C-u!QX[tW3_-ao0ٶj-n\6q+0QO+q`ϴR 0{地'a0}|? > ?|0|0}00a;Ἅ )Xx@AT|K*}\0ᾤ$A NzL aQ܇.[DI{ϫW[zGvU = †}`a߿\6G^>}>>߾vTp{ xza44ᇧ$;Dl.=A;=Z6{V|5{>4}밨/Xp{0Zㄲkiq!|c7-2/뾿_}}'t?ې/Pb㔆դRSV[e-5'-5D]xo|?}3?##-At/\yjB._~Zlᇄ}'W,#DG8GeS. 8(.3hGp/s<@Y~D-a#a89쎈\">mEhq #&:/Ѵc:g3菑>">_#/;#Dp|DtG/Gd|#@^::κWIƄF#DW&eqW9DC"bu4xOiꨜoDY;t'mw[z -u_p׾[Q]v_"tZZ%uti]'A/ZڕZ닏1UzkZM50b"#-L33㭄</׬|qȥ3gy833ea4>8dAuc^ k4xO=vֿ6+baat & [AF_Boa*gP?O1.a_G}_u%OJ?l[ʲHV[P_k᭫kئ**4i0@G!DG906>Yx4rn"3Eр NM 962\\6x1ѰS fm@l6F\ YFр<3 X9n-e`y#[ R (ar>x=euoxjE0ϧ&MQ[4J)6rGEЈ2 뮞v2"l(Whd|2 2 G౅<2gdD> W\쐋/!  <dI?qmֲΜ.Io}k =+0 0 M6# ,p >߾\0 > k|?~ >߰|77<9Ni`8w<ɆTv_F7@v:~W_LV0[(rm#}}}4w}1J$4xzi7ܜ4MH5yI,dGz Ek_f?ȤKsFx립y14¢& ut=zuˍ=q>/Font<>/ProcSet[/PDF/Text/ImageB]/ExtGState<>>>/Type/Page>> endobj 31 0 obj<>/ProcSet[/PDF/Text]/ExtGState<>>>/Type/Page>> endobj 35 0 obj<> endobj 36 0 obj<> endobj 37 0 obj<>stream HT T~wvEYEv%X?Ίi@FP1[zTԦ)?QQh1 6Vhl~ j`'9==gw̽w{R24IvKLvf&/iE63 >@)}B>y7]կ$hD_0!| FƠh2CoDLrJm@[6TQڏգFt]F-ԎF!z ^ !0 FFA4L S!!2!*X l"pI>_B| x(^0`&ƌd"Qf<3$0әf1Y1-f79t2ja8ʙH9"M6a4R#}F:nbcS9=H!H 1Q u7{L!Ř(L&MWJdJ ha4Yd HbaM#d$G*8jXp*˲KrRlR8%mEZ`K"xM 4$D$E$lPB!,&vi?AP4Uh5ӪMC&r2j6fA4m3Sv*o.MqVIL=rh:6*PRk+$i$;M&Ug3JRre4DL6[In3ܧ§?~):ѬtDhrhA6QPn1\B~;g]hbVa'abzʼ%Ji/mo87T ^וÒ9r 遳ʝԝx4*l;))Kt*)>[ܨ8w?ؐQ7a7naYpF4 6<6j8^ I**j~Ǫ?wBfWjpq4ՊC-*.0j$zmwxABխx+фoCٍ$,I*I)&ak\kָ񿐮kRzԳ]U/F)Da]jw^J۬G1X7iJVtMm8 ŗ|ؕoż.O)tumx"!]m5z*'5yyh%ݎ%Z4EqcNrjWQʽZK>#5֜Ѻ/ը7˧8Ӿ:۳?}[2ۍ5/i[r»M2LܱаڰЪ˱մ;аվ}b61(g(u׮}+ڲ^.8ϰ%'ϸZ҆⺮6j/&xMHt=[jhIOLJЧ*_lq#b&GE]|]_ҫ>*g][4g%vg=DQ 9@+2:k8M?wsXR) 2԰6T ၕ%e֒}o9 /ϊt̲P,lcX#Wbœ>QR|\aw3{ib&C\.G3,O5 'Zq 4]Gr+~{xk _\_*{Yh-of{?酓Ŵx \ߙMw3 Q^!=7 )ԩ)MR|#GhWDok!?ΘHUʏD/$LRAOA6YG3cƛ)vLz|d⍢E-\J(f6 P$Lc,]%B1R x/x}}nt5BfBcxk"h;ym7] JYĔlj`QN&.*\ 58}E\+U|~zebr,TՔnoɿ8L!<\*, XG&~52Ht'h]8׳[vP- 괩VyC{!{— tbϫ񁊇oTn:S☬R(M.O(Hå^Ŗ\-?-ܹ ~ g?=ōBMK'K UD:rrGP[ L #1 ў3)e᭕=_8+o\O?FGƖ)2hE:W뿑@?^|5j!^Xje_ 0+M!o ̀=s^!qŕDyAMTpJOfg(jf+G@+ge ;o#lM0l8f ?O"9д's&ef #4o"1yդao4=ׂ|Y"O.HQ?K+3Ru`4zz:Z M & "0jpUx1>1}ZldV2)ޠ J}<^q ^Eߵ)AxA.?MâuTfhz՟*IQ>YJg3X餽=(bli`t=p)Yuw*~hnbMCVQ Řm¨t'% m}2*/;3}JQPsl- dQ]5Ō@upz1\{f$Q$EF!*\F2jxN'Zl2ۗZn)[ʼR5:p$žFxaUt&CEHIÃw\kS|ldu a22v>ayĬk0EF]OT>;cwQkDM;ɤq5#IueW岄e?Mĝ}TWe,/ה%D46&_Eb#na͝V&FC:LiW+ X qÎ8H/VʕA q4Z[*Uc_y^<*ϫ=]?,80a!|uTg_eT` p+y ێVnH6֪/LҴi4)N.}BK*=`ɘ๐LIMHJDtQ׶/5a9plmx px:Я 䡿Fz9 :崰)8ª?ϰ~߱>q>ΩC258dԍh2ȓó1^%⠼,Fݱy;ƨf]*.<"򖻠G *p!@CF&[&jpOs*ƨWyrĆ3 >}K._hMa[O^«}IJ;,m!MyEqeaƱnQ`Gdt5ˊFER: BxEaDPt (ŠBRjt!鱬Te}={wr8F=\Or5qES2,Gl)=zR\ '$FXbxFs2w<9ih92Hz{6 lm= ~꾐3Ί0H&c~re1dJ?D DWPʍ\˜UDW'vKx J&]1~]sGGd;΍46U!P@54.tXÀ ;|&pQzFh`N4g6o >j70_ or8˦Et;F5Tqy0צU%WnÂc,[܍ )einO[ŗt6%19!1@.0xy,r.@O,<)`RAI^&qו̏GN5(Zow ?y(JaV*$zczsl:\5m$OCv5|8vHה|N6'L=+ᾇigk6h]WrWqBAGUU0xSbIRߡs&|_])vQGTਇG HHMH2O*J&w}cai xN,>Ai- nU b?_> ٳ]z6::,6P¸ա=7>0re͉6/*ɍ+!ձ.X<;;%MKL]ڿ,IKJ%$o2zDG>'\[̒ŬKL(BJ=XayDrjm|ZbWj֌/}}dI~ Vœ6{^3o;j׵[`siϿqV67-h4/`>Ix#XW:񡡭@#XQxwxS(T ΜLИ=^SD^d.S" ތf4I8QWHP %(;FnLTtm8v^,5+hL4NxIFhe=t LeŹ#(Q%%"ʏsΦ5) s'pHoh`Rw=Z[+Y|rYSR>P'KNL?"uv, e+^LC_JO-*"z۩ ~*ƴg9sdqcq ^ ~ddfV|#4,'Ɔn'"1$,i[iq wVZ'c} #LSZ%(Z^PEĥ![eJ˭}!-06hҨ(¤PZ̾R\vŠȬoٻ.-@=x=9s1аSF fx>?V{i)PW ۱qf4LK,'LgQrĒGNSX! dd|NT8 0dM0ap'TzI*`׫KO^B' 7Z)γgY& 2~S4= |5o84#uOڞ 'y9\A4ܟn' ʼn]LAeDͲ8^iML% X` j',M)DiRV(L9{@(EٶKԏ!JXz~e(|̯QbyI0ώ/beF 5rbMA=0HkgA8F:ûNQ=ۤJmF q0 )NA ۄ:cS{*-BR8^ TԎ rG#oTVa{T~ȳ{ybiu5|uK5GO/lk(4] 9y7(2=cW;{j-wOo[(ݒQt^f.-.9xs~Ngop8f;8f:ӆ[Go endstream endobj 38 0 obj<> endobj 39 0 obj<>stream H|T TT}° Ό֤5ٌ1Zk Va8"VD (A$JA'ں%Vmx.}>i}_= F 4;+)a,[)Ӓ2n_.wv;w]?m-D~a[aÆe"B!h"=Fq(P:Zȁ֡rT6mh'ڃaԉW"nrg ~!h o8t s R! rX k 6 hp NW&|1b%ƒkXMx4s2%ou7ὸ Ɲ4>/61&73?:![X<YHcTRy,f=a2co AZXn@+F.Z,#ꣲj@0ZotRuf X  TVߌV2 ib) 8a  s9Zc? aZZ^):ꧩTX<DXXto+_w?^)XxBe6t&=XTfSJx,Z{C`y: Ej)L-g@K1 kF`&H+ZZK8a\ZzN-1)K3$~Fgj5cILJX1;+aؔM$D&d&d,?_)N56SN;Q Etp};  pSP J܄pA9lR;a/!SpӐKp nU *a3l& | ]Spӛ=<|NJ|=@T@ Qu)ð _.sjuN9\?S(bNێB5lm7ն9mi8ӿhFKy9fiQŸ a33gSaG_.1?otlP:j m/ROQI_6N4q^Ti;vuaI3ULiVYXtTW\î^XdW[/jr)oiVyG8̌yKT25=5}{h'O}il^؞VkTdH\ aIx -%d!ql&+8z $F"Ӱa Q{!"R^. cm/}F!wmmܡ}90Y$gKdtĒYEbYW"žZF 6u g_UZW n anRMMW=PycKQ 鉉#K嘘ة#ϫq7Ի?/jrG'gƭ%77IS+$yn/j(VIjLP&8m']w*jUy%{H늫Gw}|MwF{宧C⤲=!\g=3$l3^5@Q\Ief,2R-D?4V4`P",6 .蝦br])Z^rWƳgEg.gMz~g9Y&Vx˕: 9?ң.MVs !u_QWuSp0 ?l"*F3hژ+>dݙ5Tn;ek(@H>H|i!2a-g_K+]+jz!Uiג6A)QR% vV$Idi*47B7%m?GE4 n@` >?$RZ6IT~ L0u2kT!BU {*wtF]19'kyL#t6#,*qtD䇺_uީ=2)'2NdHD)f,WrY4\jKttk]*ʹ,㶦j ֪-ĵ4@iϞ9e;kӞַ8l`} cw )JY\ˁ(NV5.(N=8:J{[V@+fm͐0jm !RU3Zz|!0P IσZUdE+1N1G'fSJ籒ccNfT S\nWRi"Vve(3"seEEƗ%#xro-)o4FqE&FlfJ e>6L?Y2 F׶*dMbR2f)b*l4vE ׳TDVHd q<*X6V0QeFYsڵ$n9\ /25ѲI%O8FwsQC9cx| D0f>`z.!g*nWCz߶,0 ]91¦荟K᥻Z:3T\VQMw݆JQbs#7 nKȉ R Kƪ;ŠR8].j?sE i 025xg񢋆GH[[̌Lc D2 Ċ*i1tpĜM01'k`yZUTI@Ar=2t5B[v*))Ħ#B7)Jbٿ?X Bh[u;x&StS XyS^Ck4شwbp:mg0_^.|VS.@ۗ sDxV.pGE`r(x|ACѺÊV$l4Sj1ogTOwZ+8A&aQTbubw߉>,ʄhD+sXCv|a8t|K$.*soPlw ǟA7؁ p_caaDw&>qRc*~))Қ-G_gF}8Uҗ;rKo:>]fvj5eZ%we{ ;#dco g\={Ewp _wK [0  ;!L8P܄ɔO& RҹRJNf{pWmiOd]*e4E֕ (rxApVFa,WQp u/K+n +b{UrZImL" 0J)Z ,nm +n輼rWoo`{`ͬyNc.zׂjihӬϩgzv װ2@FmUaIie|I,lܖU@{hC"fH77h#qkN|Wwƾ }rYYX"gxlШ;}OCE_}L/G3 ^třBZB|O)f\ pAB1@9ע94.'-+EC5\YptVm5Q'ɸ}(pG[dľAڵWor/<ש>!ߌ~r?1y~4QG?ئMcL;|La[lrc)E0 %1>i|,әʑ+nMEO@"/&5W5 o>ΎjV'ɩ^™"RZGB+3N" gcn>ьnj>z@6CQC3z(} Db5Hl+*ɊvEJR4M-B$DeIš ɞlɜBag\I_QC8X 6-Ƭߝ 8|!?q&)6RKk+cSd踹PzSY+'C'fٛ-cW\{w?aWon7nV`/0[ O(KeޗLlY/u`9K$/vC9^N Rh`K\J:SVZc}R8d'C|.؞Aٱi򐝠rCϖzL ĸ#i4G:[ O^%P9hX߃`jAGy(4w`2U=h~3%3)#Isel\/sO0H>boq {w^?=9+Gy's{T7!}k.)N45!t /azȁ ޞn4-PTݲn`H z$EE%YU;8%d$elؘW&V6k:!9I%)K DeGhc2SpbЇ1"`_λvFG<.(-Y5ܕ]$ =kUO:GsI]T0%gժQ ]͋yr8KdEn/-3KQ%@<ՏHtIT SJ.Wy̌ghоXON *K4H6+5}zCC?z|9mW̽%}I| <*HUڟ0>(آ| Rp|``/>UD [kS0H i3Q vP"Ԍ"pTٍ7/msͶ jyߝ&OUF$)m+S#W\ΰB.&ND'>}9z3zhPMC ;i-^QQiǠRHX,#Rz(˛)Zc^ k~T0 Xc(4RBMhSxZ$adFeal"K"c-j{;_qzebJKs\OWC" Rh\tt2A0 H}-V}\GrfJrDg_RIQ!j&2TgѮ{`P ؉&IyE;U>v!:k1wjo\_%:; BD{O(\o`&}(1z/na@x^TI~ylkKuuV7|V"\`kڛ狛,¿UfkCC S癣aµKu=,{u467$Oz "M6P3zq;xX/n=0 tu1){k\C8Nٽ+~iK "d &(m`F" l Y%+ڬȨTlG Df:ÊTR$A1!B٠sgt]cc,\8!BVojs NZCPnZ#ZM/,wst[42g=1= Z%RHA ~ V63Y4AGH+O5U)t<"=\#E Kqni%rj_ZT駙hR6Rka vjɎjJJkJ-`+Yeka>e;F2Kk2I$c0sSCݩcEݲ (H`a=|r1% p Bte~Bt𨦰>4M9*JĜe86X%<<˛sW_}K.|C~5s՗NJxB̀FfʸȺE֙08'(v6}Q0xCB윫;FȂbG^Yk~~p&驧OP5cPWvWaJs E vl*`OS( q)X@3?F//JeF ZQ9C`cAأ{txl)Zr%'oXp.)$e>9"kr~FXͮ{^cJ*v)e-9dϦ({LKkZ͏ok f*y<=ye7;\...D{@+H endstream endobj 40 0 obj<> endobj 41 0 obj<> endobj 42 0 obj<> endobj 43 0 obj<> endobj 44 0 obj<>stream H|T PSg T 4>I VEy'Xuu[C"&!:vV0"PGȜcg?o`yn`mE2e# ?#~uȗAPtV /| nazh5tA?܁j3\]{'n@z" <ȆA'&h pn'('[_[| {R85P_ ١%;aA |Upeq(/%e?"k!:WVzz7-/}>Q>þjߥc9884nK~5~nO%#ho  3$ 7'0oR,' \@ Zt"K^MSKv\6 J~՞W I(2rY1NP }2%vJ͔#/;܀=yC!v<]KJbQ~6=|g⾆Myմh9( *y6(,5߶!/O =Œj6i-R FǮϔl)(ܒFnl-YҤSOdXvieQN˜Lr¡¡(,uut% +86Jy-?yugMOew`؍cb8F0 vx F:=3bU(N^Kl5bc 攔 sjj - '"Njt+2,>x<ĺOJ b sIyKKfJ hkr^a# bAbߦeIi{")JHNɺ763[xɄ_K RBpVWƌᭋWUьHس'⏘ʬ-҂xwb4ɹ΍U<<Ǥ^͍FϹF2--mmE%K;Jk3e5-%]ߞC_p<[u|IБzY2IY+/M%L` %kltKޗlMQ6{1_؈c%g ;-$̛L ̨lݡ$R)cef1ZfΩޖk'_}4SZ 65f*=L7I<<%gbe|Չ{yJ׋D[=sfZ:rRka7ދqTh"5+ ky)D ,(e]A Ȃ*XeM %֞ 鿐F{r8sgsIrփMR:rV:!m]c}-[@[9#JIj6rGszJKr9*^ m~QYXVS*i1U cLe^{ҵ8w͎ZXr&n%5wpV7.²ʶ6327L(O=SJ [3sÑoaŅ~zAᦰb}bQ|)|JNĐ\.wZiaBL ͘J VOE أ,^BZ=~L]^$ޯklcSc^vRTVSfu X܂Y_wqD ˔anu``[|:LO)WJ PNғ(Ġ Z@&CEi72PP卍Xa &nL 5 "S4%u4=h  U'k7VvjW5z&xR!X#Jj*${3c0̡5t¶2.`Jn|-@Vg"Χ^\(>щt}[q`&An∦f.[\%ރ,I'ȱ~;8< ̠B;6qgӘم$8&ȟEvpr*܉TE¡\sYω E>[ gg#i-.hL¦4eIo鋾 Ip-3ψ%z7ޮk:QYö_I((N&hG7QM!*M+R]94_BYn.*/ -)hG4O)_S_^˟?!GMsBULT"6tRlALCrMفҷ]?|s, JIc>4oE *CF=ɴAHˌ!K)I1[ԏ ZZ Da{3(! I(rd`=baOBI(T!x6.%N|vDIr*wetqRHa${ oS[7rq? _0O@´$`ٴHF"NX2ҟ 3 ^AMi\I_s]؞mu=О`Qq2-0BBo$!P#RBbAϊFB{zЪ퍞1_wz3w7n{~#DTe9}""ȝyAp(gA8"+GD4bM *J1(KXrr8^5N/fSŠnZwKGx%%޾ =pdCK vˆ yrÉ(rO/܊z1.QwVuފMk ,yG1]l>#bCZVV%ya]6 0BOgӺG#pgȵ4r){DGd=&na(;Eb&ɭM&^ͳy3 uѿ!]|/CWa`xq-@:yx۰#: <3h 4 x"qJ<{Eh&Zӆ.v}[Amc0vkRo\]yt DtBkIhLanƢcaǢq( p :W澎7OYS7fzf kĻ |3r6wBaxI7VkƊ _qc!C+@=cńoaXCy.{/!AhW]X_ D'< $ǶADf&edb-c%q3Z0!Ûߒ0{@7e{UPw(0dvl $?%Na B)-~_W4 ?8d$FEt ȴ蔶 :_TĄuxE(fo8K#O`W*Twhd|FY6חejDärTvT*TW2~l1Dl5[Z%"MuxFtXn{;]:3uXb’x6sc\fW03Y2c]iEKoA$Y$m䰪ʹv<`G߂٤*+drLpaAa0 3<Wn%Ѕ1uf:0.A8m.؊Vcfnusu#U;fCO/":Ÿ\VܴӡR:YUGɭ؄o>Uk|,PC^o[idiQN 3zM"51z=_9;}ڊ+m#a)C1եX""I4ww5-Uݿu%|:C(a%ՠu$gge~vG++h ˗ :O gyRD[眡^O@@=݃2mkũ,h0U,gŶmaAh죰723,%b~ofhbyif5ϋO0'堠-ԕq.P]qp#iuFQG@TJEA`*H&<:hPںwC| :۱YqOluù3w3swpPO=a06PR`N\Jg"QL9='V,Szz,Q䏁 She,~ @bgDWiKsd"lPyj;Pc*OLI(/?,͋{#ҐU-j[ul)ړ++HAmKBjON& t&%Y2$*(E: \Ӂr65)99!ZDp˛~Θt΃mb4HmlAۃxg f|xxtI(^W#]҇`Ҹ}1t~59,(.G"vﲭ`n '/蔁D,k%VhLpVJ6few՞W晣΄UKչmd,6)0:8-bpaqp ^ApofZ? } 4?Y OFbu`,ٗ7s,~.~jBXB9w> endobj 46 0 obj<> endobj 47 0 obj<> endobj 48 0 obj<> endobj 49 0 obj<>/ProcSet[/PDF/Text]>>/Type/Page>> endobj 50 0 obj<> endobj 51 0 obj<> endobj 52 0 obj[50 0 R 53 0 R 55 0 R 57 0 R 59 0 R 61 0 R] endobj 53 0 obj<> endobj 54 0 obj<> endobj 55 0 obj<> endobj 56 0 obj<> endobj 57 0 obj<> endobj 58 0 obj<> endobj 59 0 obj<> endobj 60 0 obj<> endobj 61 0 obj<> endobj 62 0 obj<> endobj 63 0 obj<> endobj 64 0 obj<> endobj 65 0 obj<> endobj 66 0 obj<> endobj 67 0 obj<> endobj 68 0 obj<>stream 1 1 0.8 rg 1 i 82 720 448 36 re 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m f 0.2 0.2 0.6 RG 1 w 10 M 0 j 0 J []0 d 82 720 448 36 re 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m S 0 g BT /T1_0 1 Tf 0 Tc 0 Tw 0 Ts 100 Tz 0 Tr 10 0 0 10 220.3802 727 Tm ( 278:2157-2162, 2000. )Tj /T1_1 1 Tf -13.138 0 Td (Am J Physiol Heart Circ Physiol)Tj /T1_2 1 Tf 12 0 0 12 89 737 Tm (Paul H. Brand, Nianning Qi, Patricia J. Metting and Steven L. Britton )Tj ET 0 G 89 699 m 523 699 l 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m S 0.6 0 0 rg BT /T1_0 1 Tf 10 0 0 10 307.0895 703 Tm ( )Tj /T1_2 1 Tf -21.809 0 Td (You might find this additional information useful...)Tj 0 g /T1_0 1 Tf 6.86 -2.8 Td (9 articles, 3 of which you can access free at: )Tj 0.6 0 0 rg -6.86 0 Td (This article cites )Tj 0 g 30.212 -1.2 Td ( )Tj 0 0 1 rg -26.612 0 Td (http://ajpheart.physiology.org/cgi/content/full/278/6/H2157#BIBL)Tj 0 g -3.6 -3 Td (on the following topics: )Tj 39.984 1 Td ( )Tj 0 0 1 rg -18.695 0 Td (http://highwire.stanford.edu/lists/artbytopic.dtl)Tj 0 g -6.387 0 Td (can be found at )Tj 0.6 0 0 rg -14.902 0 Td (Medline items on this article's topics )Tj 0 g 3.6 -3.2 Td (Physiology .. Rats )Tj 0 1 TD (Physiology .. Blood Pressure )Tj 10.175 -3 Td (including high-resolution figures, can be found at: )Tj 0.6 0 0 rg -13.775 0 Td (Updated information and services )Tj 0 g 27.434 -1.2 Td ( )Tj 0 0 1 rg -23.834 0 Td (http://ajpheart.physiology.org/cgi/content/full/278/6/H2157)Tj 0 g 30.122 -2 Td ( can be found at: )Tj /T1_3 1 Tf -16.529 0 Td (AJP - Heart and Circulatory Physiology)Tj /T1_0 1 Tf -2.472 0 Td (about )Tj 0.6 0 0 rg -14.721 0 Td (Additional material and information )Tj 0 g 21.543 -1.2 Td ( )Tj 0 0 1 rg -17.943 0 Td (http://www.the-aps.org/publications/ajpheart)Tj ET 89 533 m 523 533 l 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m S 0 g BT /T1_0 1 Tf 10 0 0 10 89 508.9999 Tm (This information is current as of May 20, 2008 . )Tj T* (\240 )Tj ET 1 1 0.8 rg 82 16 448 54 re 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m f 0 g BT /T1_0 1 Tf 8 0 0 8 296.3279 22.9999 Tm ( )Tj -9.805 0 Td (http://www.the-aps.org/.)Tj -16.111 0 Td (ESSN: 1522-1539. Visit our website at )Tj 49.648 1 Td (0363-6135,)Tj -49.648 0 Td (Society, 9650 Rockville Pike, Bethesda MD 20814-3991. Copyright \251 200\ 5 by the American Physiological Society. ISSN: )Tj 0 1 TD (intact animal to the cellular, subcellular, and molecular levels. It is \ published 12 times a year \(monthly\) by the American Physiological)Tj 0 1 TD (lymphatics, including experimental and theoretical studies of cardiovasc\ ular function at all levels of organization ranging from the )Tj 16.529 1 Td ( publishes original investigations on the physiology of the heart, blood\ vessels, and)Tj /T1_3 1 Tf -16.529 0 Td (AJP - Heart and Circulatory Physiology)Tj ET 588 18 16 756 re 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m S BT /T1_4 1 Tf 0 -9 9 0 593 347.7376 Tm ( on May 20, 2008 )Tj 0 0 1 rg -10.338 0 Td (ajpheart.physiology.org)Tj 0 g -8.114 0 Td (Downloaded from )Tj ET endstream endobj 69 0 obj<> endobj 70 0 obj<> endobj 71 0 obj[69 0 R] endobj 72 0 obj<> endobj 76 0 obj<>stream 0 0 0 1 k 0 0 0 1 K 1 i 0.998 w 10 M 0 j 0 J []0 d /GS0 gs 306 713.299 m 542.477 713.299 l 554.05 713.299 563.431 703.918 563.431 692.345 c 563.431 680.773 554.05 671.391 542.477 671.391 c 69.523 671.391 l 57.95 671.391 48.569 680.773 48.569 692.345 c 48.569 703.918 57.95 713.299 69.523 713.299 c h B 189.082 680.326 362.375 26.986 re f 0 0 0 0 k BT /T1_0 1 Tf 0 Tc 0 Tw 0 Ts 100 Tz 0 Tr 35.921 0 0 35.921 189.082 680.326 Tm [(special)-366(communication)]TJ 0 0 0 1 k /T1_1 1 Tf 17.96 0 0 17.96 48.569 624.926 Tm [(A)-311(self-powered)-366(constant)-366(infusion)-366(device)]TJ 0 -1.222 TD [(for)-366(use)-366(in)-366(unrestrained)-366(rats)]TJ 9.978 0 0 9.978 96.463 563.128 Tm [(P)74(AUL)-348(H.)-366(BRAND,)-366(NIANNING)-366(QI,)-366(P)74(A)55(TRICIA)-311(J.)-366(METTING,)-311(AND)-366(STEVEN)-366(L.)-366(BRITTON)]TJ /T1_2 1 Tf 0 -1.2 TD [(Laboratory)-366(of)-366(Cardiovascular)-366(Research,)-366(Department)-366(of)-366(Physiology)-366(and)-366(Molecular)-366(Medicine,)]TJ T* [(Medical)-366(College)-366(of)-366(Ohio,)-366(T)89(oledo,)-366(Ohio)-366(43614-5804)]TJ /T1_3 1 Tf 8.98 0 0 8.98 58.547 514.474 Tm [(Brand,)-216(Paul)-216(H.,)-216(Nianning)-216(Qi,)-216(Patricia)-216(J.)-216(Metting,)-216(and)]TJ -1.111 -1.133 Td [(Steven)-329(L.)-329(Britton.)]TJ /T1_1 1 Tf 9.617 0 Td [(A)-274(self-powered)-329(constant)-329(infusion)-329(device)]TJ -9.617 -1.133 Td [(for)-251(use)-251(in)-251(unrestrained)-251(rats.)]TJ /T1_2 1 Tf 13.18 0 Td [(Am)-251(J)-251(Physiol)-251(Heart)-251(Circ)-251(Physiol)]TJ /T1_1 1 Tf -13.18 -1.133 Td [(278:)-222(H2157)-56(\261)-56(H2162,)-222(2000.\320W)55(e)-222(developed)-222(a)-222(device)-222(that)-222(deliv-)]TJ 0 -1.133 TD [(ers)-207(\257uid)-207(through)-207(a)-207(catheter)-207(at)-207(a)-207(constant)-207(rate)-207(and)-207(can)-207(be)-207(used)]TJ 0 -1.122 TD [(in)-558(conscious)-558(animals)-558(to)-558(solve)-558(a)-558(variety)-558(of)-558(problems.)-558(For)]TJ T* [(example,)-455(this)-455(device)-455(can)-455(be)-455(used)-455(for)-455(delivering)-455(drugs)-455(and)]TJ T* [(maintaining)-325(intravascular)-325(catheter)-325(patency)111(.)-325(The)-325(device)-325(pro-)]TJ T* [(vides)-317(infusions)-317(at)-317(low)-317(\257ows)-317(\(1.0)-56(\261)-56(1.5)-317(ml/day\),)-317(so)-317(that)-317(experi-)]TJ 0 -1.122 TD [(mental)-455(agents)-455(may)-455(be)-455(administered)-455(with)-455(minimal)-455(volume)]TJ T* [(loading)-420(of)-420(the)-420(rat.)-366(Arterial)-420(and)-420(venous)-420(catheter)-420(patency)-420(is)]TJ -0.001 Tc T* [(maintained)-198(by)-198(infusion)-198(of)-198(heparinized)-198(saline)-198(through)-198(indwell-)]TJ 0 Tc T* [(ing)-301(catheters)-301(attached)-301(to)-301(the)-301(device.)-301(The)-301(catheters)-301(exit)-301(from)]TJ T* [(the)-384(rat)-384(in)-384(the)-384(intrascapular)-384(area)-384(and)-384(are)-384(routed)-384(through)-384(a)]TJ T* [(protective)-264(spring)-264(to)-264(the)-264(device,)-264(which)-264(is)-264(suspended)-264(above)-264(the)]TJ 0 -1.122 TD [(cage.)-237(The)-237(catheters)-237(may)-237(be)-237(attached)-237(to)-237(pressure)-237(transducers,)]TJ 0 -1.122 TD [(blood)-443(may)-443(be)-443(sampled,)-443(and)-443(injections)-443(or)-443(infusions)-443(may)-443(be)]TJ T* [(made)-621(without)-621(disturbing)-621(the)-621(rat.)-621(Because)-621(the)-621(device)-621(is)]TJ T* [(self-contained,)-258(it)-258(can)-258(be)-258(suspended)-258(by)-258(a)-258(\257uid-free)-258(swivel)-258(that)]TJ T* [(rotates)-407(through)-407(360\311,)-407(providing)-407(minimal)-407(restraint.)-407(The)-407(de-)]TJ T* [(vice)-582(has)-582(been)-582(used)-582(successfully)-582(to)-582(measure)-582(arterial)-582(and)]TJ T* [(central)-233(venous)-233(blood)-233(pressures)-233(in)-233(two)-233(studies)-233(using)-233(rats.)]TJ -0.004 Tc 0 -1.678 TD [(chronic)-195(instrumentation;)-195(blood)-195(pressure)-195(measurement;)-195(vascu-)]TJ 0 Tc 0 -1.122 TD [(lar)-233(access;)-233(catheterization)]TJ ET q 66.529 264.234 215.135 3.002 re W n q 0 g 215.759903 0 0 3.6000061 66.5290985 264.2342987 cm BI /W 899 /H 15 /IM true /BPC 1 ID ? ? EI Q Q BT /T1_1 1 Tf 7.441 0 0 7.441 48.569 236.509 Tm [(DIRECT)-758(MEASUREMENT)]TJ 9.978 0 0 9.978 152.438 236.509 Tm [(of)-565(arterial)-565(blood)-565(pressure)-565(and)]TJ -10.41 -1.11 Td [(infusion)-253(of)-253(experimental)-253(agents)-253(over)-253(prolonged)-253(periods)]TJ 0 -1.11 TD [(is)-463(often)-463(required)-463(in)-463(studies)-463(in)-463(which)-463(small)-463(animals,)]TJ 0 -1.11 TD [(such)-482(as)-482(rats,)-482(have)-482(unrestrained)-482(movement)-482(and)-482(free)]TJ 0 -1.11 TD [(access)-243(to)-243(food)-243(and)-243(water)74(.)-243(Direct)-243(pressure)-243(measurement)]TJ T* [(and)-500(infusions)-500(require)-500(indwelling)-500(vascular)-500(catheters.)]TJ T* [(The)-276(primary)-276(di)10(f)28(f)28(iculty)-276(that)-276(arises)-276(in)-276(maintaining)-276(func-)]TJ -0.001 Tc T* [(tional)-198(indwelling)-198(vascular)-198(catheters)-198(is)-198(thrombus)-198(forma-)]TJ 0 Tc 0 -1.11 TD [(tion)-458(at)-458(the)-458(catheter)-458(tip)-458(\(1,)-458(10\).)-458(Thrombus)-458(formation)]TJ 0 -1.11 TD [(may)-234(be)-234(prevented)-234(or)-234(delayed)-234(by)-234(infusion)-234(of)-234(heparinized)]TJ T* [(saline,)-301(along)-301(with)-301(infusion)-301(of)-301(any)-301(required)-301(experimen-)]TJ T* [(tal)-344(agents.)-344(One)-344(approach)-344(to)-344(providing)-344(prolonged)-344(infu-)]TJ 26.4 39.998 Td [(sions)-441(in)-441(unrestrained)-441(animals)-441(is)-441(to)-441(use)-441(360\311)-441(swivels)]TJ 0 -1.15 TD [(with)-500(\257uid)-500(channels)-500(\(5,)-500(6\))-500(to)-500(compensate)-500(for)-500(animal)]TJ T* [(movement.)-312(This)-312(approach)-312(can)-312(be)-312(technically)-312(challeng-)]TJ T* [(ing)-412(and)-412(expensive.)-412(Many)-412(studies)-412(have)-412(used)-412(commer)18(-)]TJ T* [(cially)-423(available)-423(swivel)-423(systems)-423(that)-423(allow)-423(free)-423(move-)]TJ T* [(ment)-484(of)-484(the)-483(rat)-484(during)-483(infusion)-483(via)-483(a)-483(pump)-483(located)]TJ T* [(external)-393(to)-393(the)-393(animal')37(s)-393(cage)-393(\(3,)-393(7\).)-393(Despite)-393(the)-393(wide)]TJ T* [(use)-275(of)-275(commercially)-275(available)-275(swivel)-275(devices,)-275(our)-275(expe-)]TJ T* [(rience)-354(with)-354(these)-354(products)-354(has)-354(proven)-354(to)-354(be)-354(less)-354(than)]TJ T* [(satisfactory)-634(under)-634(many)-634(circumstances.)-634(The)-634(major)]TJ T* [(problem)-233(is)-233(stabilizing)-233(the)-233(interface)-233(between)-233(the)-233(animal)]TJ T* [(and)-233(the)-233(catheter)-233(system.)-233(Because)-233(of)-233(variable)-233(rotational)]TJ 0 -1.14 TD [(friction)-472(of)-472(the)-472(swivel)-472(systems,)-472(complicated)-472(tethering)]TJ T* [(and)-218(harness)-218(systems)-218(are)-218(required)-218(to)-218(keep)-218(the)-218(skin)-218(from)]TJ T* [(getting)-233(distorted.)]TJ 1 -1.14 Td [(Another)-429(approach)-429(to)-429(compensate)-429(for)-429(animal)-429(move-)]TJ -1 -1.14 Td [(ment)-247(is)-247(to)-247(construct)-247(a)-247(rotating)-247(cage.)-247(Rotating)-247(cages)-247(are)]TJ T* [(complicated)-396(to)-396(construct)-396(\(8\))-396(and)-396(result)-396(in)-396(the)-396(animal)]TJ 0 -1.14 TD [(being)-452(studied)-452(in)-452(an)-452(unusual)-452(environment,)-452(a)-452(circum-)]TJ T* [(stance)-233(that)-233(can)-233(in\257uence)-233(the)-233(results)-233(by)-233(itself.)]TJ 1 -1.14 Td [(In)-262(two)-262(recent)-262(studies,)-262(we)-262(evaluated)-262(the)-262(development)]TJ -1 -1.14 Td [(of)-398(hypertension)-398(in)-398(Dahl)-398(salt-sensitive)-398(rats)-398(\(4,)-398(9\).)-398(For)]TJ T* [(these)-606(studies,)-606(a)-606(simple)-606(and)-606(reliable)-606(approach)-606(was)]TJ 0 -1.14 TD [(devised)-386(to)-386(measure)-386(arterial)-386(and)-386(central)-386(venous)-386(blood)]TJ T* [(pressures)-649(directly)-649(over)-649(prolonged)-649(periods)-649(in)-649(unre-)]TJ T* [(strained,)-399(chronically)-399(instrumented)-399(rats.)-399(W)37(ith)-399(the)-399(de-)]TJ T* [(vice)-220(we)-220(created,)-220(catheter)-220(patency)-220(is)-220(maintained)-220(by)-220(slow)]TJ 0 -1.14 TD [(infusion)-450(of)-450(heparinized)-450(saline.)-450(Because)-450(the)-450(device)-450(is)]TJ T* [(self-powered)-448(and)-448(self-contained,)-448(it)-448(can)-448(be)-448(suspended)]TJ T* [(above)-251(the)-251(rat')37(s)-251(cage)-251(by)-251(a)-251(\257uid-free)-251(swivel,)-251(which)-251(allows)]TJ T* [(trouble-free)-578(rotation)-578(through)-578(360\311.)-578(This)-578(device)-578(has)]TJ T* [(operated)-308(reliably)-308(in)-308(over)-308(100)-308(rats)-308(to)-308(maintain)-308(patency)]TJ T* [(of)-233(arterial)-233(and)-233(venous)-233(catheters.)]TJ 1 -1.14 Td [(Because)-360(this)-360(device)-360(is)-360(capable)-360(of)-360(infusing)-360(\257uid)-360(at)-360(a)]TJ -1 -1.14 Td [(constant)-280(rate,)-280(it)-280(can)-280(also)-280(be)-280(used)-280(to)-280(provide)-280(infusion)-280(of)]TJ 0 -1.14 TD [(experimental)-328(agents.)-328(Because)-328(of)-328(the)-328(low)-328(cost,)-328(reliabil-)]TJ T* [(ity)111(,)-574(ease)-574(of)-574(construction)-574(and)-574(use,)-574(and)-574(lack)-574(of)-574(skin)]TJ T* [(distortion)-259(with)-259(this)-259(device,)-259(and)-259(its)-259(potential)-259(for)-259(provid-)]TJ 0.009 Tc T* [(ing)-675(constant)-675(infusion)-675(of)-675(experimental)-675(agents,)-675(we)]TJ 0 Tc 0 -1.14 TD [(thought)-485(it)-485(would)-485(be)-485(of)-485(service)-485(to)-485(provide)-485(a)-485(detailed)]TJ T* [(description)-233(of)-233(its)-233(design,)-233(characteristics,)-233(and)-233(use.)]TJ ET 0.499 w 48.569 94.382 m 300.013 94.382 l S BT /T1_1 1 Tf 7.982 0 0 7.982 56.551 84.688 Tm [(The)-263(costs)-263(of)-263(publication)-263(of)-263(this)-263(article)-263(were)-263(defrayed)-263(in)-263(part)-263(by)-263(the)]TJ -1 -1.125 Td [(payment)-566(of)-566(page)-566(charges.)-566(The)-566(article)-566(must)-566(therefore)-566(be)-566(hereby)]TJ 0 -1.125 TD [(marked)-369(`)18(`)]TJ /T1_2 1 Tf 4.314 0 Td (advertisement)Tj /T1_1 1 Tf 6.46 0 Td [(')18(')-314(in)-369(accordance)-369(with)-369(18)-369(U.S.C.)-369(Section)-369(1734)]TJ -10.775 -1.125 Td [(solely)-233(to)-233(indicate)-233(this)-233(fact.)]TJ /T1_2 1 Tf 49.688 86.254 Td [(Am)-249(J)-249(Physiol)-249(Heart)-249(Circ)-249(Physiol)]TJ /T1_1 1 Tf 2.811 -1.125 Td [(278:)-249(H2157)-56(\261)-56(H2162,)-249(2000.)]TJ -37.46 -87.379 Td [(0363-6135/00)-366($5.00)-366(Copyright)]TJ /T1_4 1 Tf 9.578 0 0 9.578 283.537 37.395 Tm (r)Tj /T1_1 1 Tf 7.982 0 0 7.982 291.783 39.787 Tm [(2000)-366(the)-311(American)-366(Physiological)-366(Society)-11974(H2157)]TJ -30.469 0 Td [(http://www)92(.ajpheart.org)]TJ ET 0 G 1 w /GS1 gs 588 18 16 756 re 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m S 0 g /GS1 gs BT /T1_5 1 Tf 0 -9 9 0 593 347.7376 Tm ( on May 20, 2008 )Tj 0 0 1 rg ET /GS1 gs BT /T1_5 1 Tf 0 -9 9 0 593 440.7795 Tm (ajpheart.physiology.org)Tj 0 g ET /GS1 gs BT /T1_5 1 Tf 0 -9 9 0 593 513.8053 Tm (Downloaded from )Tj ET endstream endobj 77 0 obj<> endobj 78 0 obj<> endobj 79 0 obj[77 0 R] endobj 80 0 obj<> endobj 84 0 obj<>stream 0 0 0 1 k 1 i /GS0 gs BT /T1_0 1 Tf 0 Tc 0 Tw 0 Ts 100 Tz 0 Tr 7.982 0 0 7.982 48.569 722.313 Tm (METHODS)Tj /T1_1 1 Tf 8.98 0 0 8.98 48.569 705.056 Tm [(Self-Powered)-249(Infusion)-249(Device)]TJ /T1_2 1 Tf 1.111 -1.889 Td [(The)-546(self-powered)-546(infusion)-546(device)-546(illustrated)-546(in)-546(Fig.)-546(1)]TJ /T1_1 1 Tf 26.19 0 Td (A)Tj /T1_2 1 Tf -27.301 -1.111 Td [(consists)-401(of)-401(a)-401(latex)-401(rubber)-401(bulb)-401(that)-401(acts)-401(as)-401(a)-401(pressure)-401(and)]TJ 0 -1.111 TD [(\257uid)-211(reservoir)74(,)-211(a)-211(syringe)-211(\256lter)-211(to)-211(remove)-211(bacteria)-211(and)-211(particu-)]TJ T* [(late)-349(matter)74(,)-349(and)-349(a)-349(hydraulic)-349(resistor)-349(to)-349(set)-349(the)-349(level)-349(of)-349(\257ow)92(.)]TJ T* [(The)-354(rubber)-354(bulb)-354(of)-354(the)-354(type)-354(commonly)-354(used)-354(for)-354(pipetting)-354(\(2)]TJ T* [(ml;)-288(New)-288(Jersey)-288(Rubber\))-288(is)-288(connected)-288(in)-288(series)-288(with)-288(a)-288(0.2-m)]TJ T* [(syringe)-276(\256lter)-276(\(product)-276(no.)-276(4192)-276(sterile)-222(Acrodisc)-276(syringe)-276(\256lter)74(,)]TJ T* [(Gelman)-445(Sciences,)-390(Ann)-390(Arbor)74(,)-445(MI\))-445(and)-445(a)-445(hydraulic)-445(resistor)]TJ T* [(through)-366(a)-366(nylon)-366(three-way)-366(stopcock)-366(\(K75,)-366(Baxter)74(,)-366(V)92(alencia,)]TJ T* [(CA\).)-489(The)-489(resistor)-489(is)-489(manufactured)-489(in)-489(our)-489(laboratory)-489(from)]TJ T* [(fused)-258(silica)-258(capillary)-258(tubing)-258(\(25)-258(m)-258(ID;)-258(Scienti\256c)-258(Instrument)]TJ T* [(Services,)-476(Ringoes,)-476(NJ\).)-476(The)-476(tubing)-476(is)-476(\257amed)-476(in)-476(a)-476(Bunsen)]TJ T* [(burner)-379(to)-379(remove)-379(its)-379(outer)-379(coating,)-379(and)-379(a)-379(section)]TJ /T1_3 1 Tf 23.598 0 Td (,)Tj /T1_2 1 Tf 0.379 Tc 0.833 0 Td [(4c)379(mi)379(n)]TJ 0 Tc -24.431 -1.111 Td [(length)-337(is)-337(embedded)-337(within)-337(a)-337(21-gauge)-337(blunt-tip)-337(hypodermic)]TJ T* [(needle)-204(\(305167,)-204(Becton-Dickinson,)-204(Franklin)-204(Lanes,)-204(NJ\))-204(using)]TJ T* [(Dow-Corning)-233(Silastic)-233(glue.)]TJ 30.444 20.389 Td [(During)-287(use,)-287(each)-287(vascular)-287(catheter)-287(is)-287(connected)-287(to)-287(its)-287(own)]TJ -1.111 -1.111 Td [(infusion)-406(device.)-406(T)92(o)-406(provide)-406(a)-406(low-resistance)-406(route)-406(for)-406(blood)]TJ 0 -1.111 TD [(sampling)-295(and)-295(blood)-295(pressure)-295(recording)-295(through)-295(the)-295(vascular)]TJ T* [(catheters,)-272(a)-272(second)-272(three-way)-272(stopcock)-272(is)-272(connected)-272(in)-272(paral-)]TJ T* [(lel)-393(to)-393(the)-393(stopcock)-393(holding)-393(the)-393(resistor)-393(\(Fig.)-393(1)]TJ /T1_1 1 Tf 21.733 0 Td (A)Tj /T1_2 1 Tf 0.704 0 Td [(\))-393(by)-393(a)-393(short)]TJ -22.437 -1.111 Td [(length)-298(of)-298(T)52(ygon)-298(tubing)-298(to)-298(provide)-298(mechanical)-298(stability)-298(and)-298(a)]TJ T* [(three-way)-343(T)-343(connector)-343(to)-343(direct)-343(\257uid)-343(movement)-343(\(20)-343(gauge/3)]TJ 0 -1.111 TD [(way)111(,)-546(Small)-546(Parts,)-546(Logansport,)-546(IN\).)-546(During)-546(infusions,)-546(the)]TJ 0 -1.111 TD [(stopcock)-309(for)-309(the)-309(low-resistance)-309(route)-309(is)-309(closed)-309(to)-309(the)-309(animal,)]TJ T* [(whereas)-387(during)-387(pressure)-387(measurements)-387(or)-387(blood)-387(sampling,)]TJ T* [(the)-280(stopcock)-280(to)-280(the)-280(hydraulic)-280(resistor)-280(is)-280(closed)-280(to)-280(the)-280(animal.)]TJ T* [(The)-337(device)-337(is)-337(suspended)-337(from)-337(a)-337(support)-337(arm)-337(above)-337(the)-337(rat')37(s)]TJ T* [(cage)-236(through)-236(a)-236(ball-bearing)-236(\256shing)-236(line-type)-236(swivel)-236(\(obtained)]TJ T* [(from)-262(a)-262(local)-262(sporting)-262(goods)-262(store\))-262(that)-262(rotates)-262(freely)-262(in)-262(either)]TJ T* [(direction)-233(through)-233(360\311\(Fig.)-233(1)]TJ /T1_1 1 Tf 13.246 0 Td (B)Tj /T1_2 1 Tf 0.722 0 Td (\).)Tj /T1_1 1 Tf -13.968 -2.021 Td [(In)-249(V)62(itro)-249(T)89(ests)-249(of)-249(Flow)-249(and)-249(Pressure)-249(Characteristics)]TJ 1.111 -1.889 Td [(Constancy)-385(of)-385(\257uid)-385(delivery)55(.)]TJ /T1_2 1 Tf 13.149 0 Td [(T)92(o)-385(determine)-385(the)-385(\257ow)-385(versus)]TJ -14.26 -1.111 Td [(time)-401(characteristics)-401(of)-401(the)-401(\257uid)-401(delivery)-401(device,)-401(six)-401(similar)]TJ T* [(devices)-250(with)-250(45)-250(mm)]TJ /T1_4 1 Tf 9.314 0 Td (3)Tj /T1_2 1 Tf 1.083 0 Td [(25)-250(m)-250(ID)-250(capillary)-250(tube)-250(resistors)-250(were)]TJ -10.397 -1.111 Td [(prepared,)-219(the)-219(bulb)-219(was)-219(\256lled)-219(with)]TJ /T1_3 1 Tf 15.5 0 Td (,)Tj /T1_2 1 Tf 0.833 0 Td [(10)-219(ml)-219(of)-219(water)74(,)-219(tared)-219(with)]TJ -16.333 -1.111 Td [(an)-444(analytic)-444(balance)-444(sensitive)-444(to)-444(0.00001)-444(g,)-444(and)-444(allowed)-444(to)]TJ T* [(deliver)-204(water)-204(freely)111(.)-204(W)55(eight)-204(was)-204(monitored)-204(once)-204(or)-204(twice)-204(daily)]TJ T* [(for)-258(67)-258(h)-258(to)-258(determine)-258(the)-258(decrease)-258(in)-258(weight)-258(with)-258(time)-258(due)-258(to)]TJ T* [(delivery)-233(of)-233(water)-233(from)-233(the)-233(devices.)]TJ /T1_1 1 Tf 1.111 -1.111 Td [(Reproducibility)-216(of)-216(\257ow)37(.)]TJ /T1_2 1 Tf 10.718 0 Td [(Five)-216(similar)-216(devices)-216(were)-216(prepared,)]TJ -11.829 -1.111 Td [(again)-308(with)-308(45)-308(mm)]TJ /T1_4 1 Tf 8.788 0 Td (3)Tj /T1_2 1 Tf 1.141 0 Td [(25)-308(m)-308(ID)-308(resistors,)-308(\256lled)-308(with)-308(10)-308(ml)-308(of)]TJ -9.929 -1.111 Td [(water)74(,)-642(and)-642(tared)-642(as)-642(above.)-642(Flow)-642(from)-642(each)-642(device)-642(was)]TJ T* [(measured)-256(as)-256(the)-256(decrease)-256(in)-256(its)-256(weight)-256(during)-256(three)-256(consecu-)]TJ T* (tive)Tj /T1_3 1 Tf 1.974 0 Td (,)Tj /T1_2 1 Tf 0.833 0 Td [(24-h)-233(periods.)]TJ /T1_1 1 Tf -1.695 -1.111 Td [(Flow)-248(versus)-248(length)-248(of)-248(resistor)129(.)]TJ /T1_2 1 Tf 13.7 0 Td [(The)-248(utility)-248(of)-248(this)-248(device)-248(as)-248(a)]TJ -14.812 -1.111 Td [(constant)-402(\257ow)-402(delivery)-402(system)-402(may)-402(be)-402(improved)-402(if)-402(di)10(f)28(ferent)]TJ T* [(\257ows)-467(can)-467(be)-467(readily)-467(achieved.)-467(One)-467(way)-467(in)-467(which)-467(variable)]TJ T* [(\257ows)-434(can)-434(be)-434(obtained)-434(is)-434(by)-434(using)-434(devices)-434(with)-434(resistors)-434(of)]TJ T* [(di)10(f)28(ferent)-222(lengths.)-222(T)92(o)-222(test)-222(the)-222(e)10(f)28(fect)-222(of)-222(the)-222(length)-222(of)-222(the)-222(resistor)]TJ T* [(on)-246(\257ow)92(,)-246(seven)-246(devices)-246(were)-246(prepared)-246(with)-246(25-m)-246(ID)-246(capillary)]TJ T* [(tube)-253(resistors)-253(of)-253(lengths)-253(varying)-253(from)-253(30)-253(to)-253(47)-253(mm.)-253(Flow)-253(was)]TJ T* [(determined)-271(over)-271(24)-271(h)-271(from)-271(each)-271(of)-271(these)-271(devices)-271(as)-271(described)]TJ T* (above.)Tj /T1_1 1 Tf 1.111 -1.111 Td [(Constancy)-490(of)-490(pressure)-490(head.)]TJ /T1_2 1 Tf 13.916 0 Td [(Given)-490(that)-490(the)-490(devices)-490(are)]TJ -15.027 -1.111 Td [(prepared)-334(with)-334(\256xed)-334(resistors,)-334(constancy)-334(of)-334(\257ow)-334(implies)-334(that)]TJ T* [(there)-577(must)-577(be)-577(a)-577(constant)-577(pressure)-577(source.)-577(The)-577(pressure)]TJ T* [(source)-371(is)-371(the)-371(rubber)18(-nipple)-371(bulb)-371(\257uid)-371(reservoir)74(.)-371(W)55(e)-371(hypoth-)]TJ T* [(esized)-282(that)-282(a)-282(constant)-282(pressure)-282(head)-282(may)-282(be)-282(provided)-282(by)-282(the)]TJ T* [(bulb)-579(following)-579(the)-579(law)-579(of)-579(Laplace)-579(\(2\):)-579(pressure)]TJ /T1_4 1 Tf 23.965 0 Td (5)Tj /T1_2 1 Tf 1.412 0 Td ([\(wall)Tj -25.377 -1.111 Td [(stress\)\(wall)-233(thickness\)]/radius.)]TJ 1.111 -1.111 Td [(Preliminary)-246(observations)-246(suggested)-246(that)-246(pressure)-246(declines)]TJ -1.111 -1.111 Td [(very)-503(little)-503(as)-503(the)-503(bulb)-503(delivers)-503(\257uid)-503(through)-503(the)-503(resistor)74(,)]TJ T* [(suggesting)-552(that)-552(the)-552(product)-552(of)-552(wall)-552(stress)-552(and)-552(thickness)]TJ T* [(declines)-434(in)-434(proportion)-434(to)-434(the)-434(decline)-434(in)-434(radius)-434(as)-434(the)-434(\257uid)]TJ T* [(leaves)-527(the)-527(bulb.)-527(T)92(o)-527(characterize)-527(simultaneous)-527(changes)-527(in)]TJ T* [(pressure)-345(and)-345(volume)-345(in)-345(the)-345(bulb,)-345(a)-345(bulb)-345(was)-345(attached)-345(via)-345(a)]TJ -0.004 Tc T* [(four)17(-way)-196(stopcock)-196(to)-196(a)-196(syringe)-196(mounted)-196(in)-196(a)-196(constant)-196(infusion-)]TJ 0 Tc T* [(withdrawal)-421(pump)-421(\(Harvard)-366(Apparatus,)-421(South)-421(Natick,)-421(MA\))]TJ T* [(and)-350(to)-350(a)-350(Statham)-350(23)-350(Db)-350(pressure)-350(transducer)74(.)-350(The)-350(bulb)-350(was)]TJ T* [(\256lled)-371(with)]TJ /T1_3 1 Tf 5.149 0 Td (,)Tj /T1_2 1 Tf 0.833 0 Td [(12)-371(ml)-371(of)-371(water)74(.)-316(A)-316(12-ml)-371(syringe)-371(was)-371(tared)-371(and)]TJ -5.982 -1.111 Td [(placed)-318(in)-318(the)-318(syringe)-318(pump,)-318(and)-318(water)-318(was)-318(withdrawn)-318(from)]TJ T* [(the)-374(bulb)-374(into)-374(the)-374(syringe)-374(by)-374(the)-374(pump)-374(at)-374(a)-374(nominal)-374(rate)-374(of)]TJ T* [(0.12)-206(ml/min.)-151(After)]TJ /T1_3 1 Tf 8.471 0 Td (,)Tj /T1_2 1 Tf 0.833 0 Td [(10)-206(ml)-206(had)-206(been)-206(withdrawn)-206(from)-206(the)-206(bulb,)]TJ -9.304 -1.111 Td [(the)-299(experiment)-299(was)-299(stopped,)-299(and)-299(the)-299(syringe)-299(was)-299(reweighed)]TJ 0 -1.111 TD [(to)-454(determine)-454(the)-454(actual)-454(volume)-454(withdrawn)-454(from)-454(the)-454(bulb.)]TJ T* [(While)-373(water)-373(was)-373(withdrawn)-373(from)-373(the)-373(bulb,)-373(the)-373(pressure)-373(in)]TJ T* [(the)-435(bulb)-435(was)-435(determined)-435(from)-435(the)-435(voltage)-435(signal)-435(from)-435(the)]TJ T* [(transducer)74(.)-580(The)-580(signal)-580(was)-580(ampli\256ed)-580(on)-580(a)-580(Sensormedics)]TJ T* [(R-61)55(1)-283(polygraph,)-283(sampled)-283(at)-283(300)-283(Hz,)-283(and)-283(then)-283(digitized)-283(by)-283(a)]TJ T* [(Data)-591(T)62(ranslation)-591(analog-to-digital)-591(board)-591(\(DT)-591(2801,)-591(Data)]TJ T* [(T)62(ranslation,)-242(Marlboro,)-242(MA\))-242(The)-242(digital)-242(signals)-242(were)-242(sampled)]TJ ET q 48.569 320.57 252.159 198.125 re W n q 252 0 0 198.7200012 48.5688019 320.5700989 cm /Im0 Do Q Q q 48.569 96.007 252.159 219.138 re W n q 252 0 0 219.3300018 48.5688019 96.0066071 cm /Im1 Do Q Q BT /T1_2 1 Tf 7.982 0 0 7.982 48.569 84.688 Tm [(Fig.)-319(1.)]TJ /T1_1 1 Tf 3.505 0 Td (A)Tj /T1_2 1 Tf 0.704 0 Td [(:)-319(self-powered)-318(constant)-318(infusion)-318(device)-318(with)-318(low-resistance)]TJ -4.209 -1.125 Td [(pathway)-406(for)-406(blood)-406(pressure)-406(measurements)-406(and)-406(blood)-406(sampling.)]TJ /T1_1 1 Tf 30.505 0 Td (B)Tj /T1_2 1 Tf 0.722 0 Td (:)Tj -31.227 -1.125 Td [(setup)-448(for)-448(chronic)-448(intravascular)-448(infusion)-448(in)-448(unrestrained)-448(rats.)-448(Rat)]TJ 0 -1.125 TD [(shown)-233(in)-233(home)-233(cage.)]TJ 9.978 0 0 9.978 48.569 744.772 Tm (H2158)Tj 7.982 0 0 7.982 207.629 744.772 Tm [(SELF-POWERED)-366(CONST)55(ANT)-366(INFUSION)-366(DEVICE)]TJ ET 0 G 1 w 10 M 0 j 0 J []0 d /GS1 gs 588 18 16 756 re 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m S 0 g /GS1 gs BT /T1_5 1 Tf 0 -9 9 0 593 347.7376 Tm ( on May 20, 2008 )Tj 0 0 1 rg ET /GS1 gs BT /T1_5 1 Tf 0 -9 9 0 593 440.7795 Tm (ajpheart.physiology.org)Tj 0 g ET /GS1 gs BT /T1_5 1 Tf 0 -9 9 0 593 513.8053 Tm (Downloaded from )Tj ET endstream endobj 85 0 obj<> endobj 86 0 obj<> endobj 87 0 obj[85 0 R] endobj 88 0 obj<> endobj 92 0 obj<>stream 0 0 0 1 k 1 i /GS0 gs BT /T1_0 1 Tf 0 Tc 0 Tw 0 Ts 100 Tz 0 Tr 8.98 0 0 8.98 48.569 530.776 Tm [(at)-491(0.1)-491(Hz)-491(by)-491(a)-491(PC)-491(\(Dell)-491(Dimension)-491(P75t,)-491(Dell)-491(Computer)74(,)]TJ 0 -1.111 TD [(Round)-200(Rock,)-200(TX\))-200(using)-200(Labtech)-200(Notebook)-200(V8.0)-200(\(Andover)74(,)-200(MA\))]TJ T* [(software)-412(and)-412(stored)-412(on)-412(the)-412(hard)-412(drive)-412(for)-412(o)10(f)28(f-line)-412(analysis.)]TJ T* [(One)-394(to)-394(four)-394(repeat)-394(simultaneous)-394(measures)-394(of)-394(pressure)-394(and)]TJ T* [(volume)-599(were)-599(made)-599(on)-599(each)-599(of)-599(four)-599(bulbs.)-599(T)92(o)-599(determine)]TJ 0 -1.111 TD [(absolute)-535(pressures,)-535(the)-535(transducer)-535(was)-535(calibrated)-535(with)-535(a)]TJ T* [(mercury)-233(manometer)74(.)]TJ /T1_1 1 Tf 0 -1.889 TD [(In)-249(V)62(ivo)-249(Experiments:)-249(Use)-249(of)-249(Flow)-249(Device)-249(to)-249(Maintain)]TJ 0 -1.111 TD [(Catheter)-249(Patency)]TJ 1.111 -1.889 Td [(Animal)-351(preparation)-351(and)-351(experimental)-351(setup.)]TJ /T1_0 1 Tf 21.061 0 Td [(The)-351(animals)]TJ -22.172 -1.111 Td [(used)-563(in)-563(this)-563(study)-563(were)-563(John)-563(Rapp)-563(inbred)-563(salt-sensitive)]TJ T* [(\(SS/Jr\))-376(and)-376(salt-resistant)-376(\(SR/Jr\))-376(rats.)-376(Results)-376(of)-376(studies)-376(of)]TJ T* [(the)-509(mechanisms)-509(leading)-509(to)-509(hypertension)-509(using)-509(these)-509(rats)]TJ T* [(have)-595(been)-595(published)-595(previously)-595(\(8,)-595(9\).)-540(All)-595(animals)-595(were)]TJ 0 -1.111 TD [(anesthetized)-230(with)-230(a)-230(solution)-230(of)-230(ketamine)-230(\(80)-230(mg/kg\),)-230(xylazine)]TJ 0 -1.111 TD [(\(12)-236(mg/kg\),)-236(and)-236(atropine)-236(\(0.032)-236(mg/kg\))-236(via)-236(an)-236(intraperitoneal)]TJ T* [(injection.)-453(T)71(wo)-453(micro-Renathane)-453(catheters)-453(\(0.040)-453(in.)-453(OD)]TJ /T1_2 1 Tf 27.172 0 Td (3)Tj /T1_0 1 Tf -27.172 -1.111 Td [(0.025)-411(in.)-411(ID;)-411(Braintree)-411(Scienti\256c,)-411(Braintree,)-411(MA\))-411(extended)]TJ T* [(with)-249(T)52(ygon)-249(plastic)-249(tubing)-249(\(0.60)-249(in.)-249(OD)]TJ /T1_2 1 Tf 17.749 0 Td (3)Tj /T1_0 1 Tf 1.082 0 Td [(0.020)-249(in.)-249(ID;)-249(Norton)]TJ -18.831 -1.111 Td [(Performance)-253(Plastic,)-198(Akron,)-253(OH\))-253(were)-253(inserted)-253(into)-253(the)-253(right)]TJ T* [(carotid)-283(artery)-283(for)]TJ /T1_3 1 Tf 8.219 0 Td (,)Tj /T1_0 1 Tf 0.833 0 Td [(2.0)-283(cm)-283(and)-283(into)-283(the)-283(right)-283(jugular)-283(vein)-283(for)]TJ /T1_3 1 Tf -9.052 -1.111 Td (,)Tj /T1_0 1 Tf 0.833 0 Td [(3.2)-293(cm,)-293(respectively)111(,)-293(for)-293(the)-293(direct)-293(measurement)-293(of)-293(arterial)]TJ -0.001 Tc -0.833 -1.111 Td [(pressure,)-198(central)-198(venous)-198(pressure,)-198(collection)-198(of)-198(blood)-198(samples,)]TJ 0 Tc T* [(and)-439(injection)-439(of)-439(experimental)-439(agents.)-439(The)-439(free)-439(ends)-439(of)-439(the)]TJ T* [(tubing)-325(were)-325(tunneled)-325(subcutaneously)-325(to)-325(exit)-325(the)-325(skin)-325(at)-325(the)]TJ T* [(intrascapular)-271(region)-271(through)-271(a)-271(\257anged)-271(entry)-271(portal)-271(that)-271(was)]TJ T* [(\256xed)-447(beneath)-447(the)-447(skin.)-447(The)-447(entry)-447(portal)-447(was)-447(made)-447(in)-447(our)]TJ T* [(laboratory)-266(from)-266(stainless)-266(steel)-266(tubing)-266(\(4)-266(mm)-266(OD)]TJ /T1_2 1 Tf 22.577 0 Td (3)Tj /T1_0 1 Tf 0.266 Tc 1.099 0 Td [(3m)266(mI)266(D)266(;)]TJ 0 Tc -23.676 -1.111 Td [(Small)-437(Parts,)-437(Logansport,)-437(IN\).)-437(The)-437(exteriorized)-437(tubing)-437(was)]TJ T* [(routed)-232(out)-232(of)-232(the)-232(animal)-232(cage)-232(through)-232(a)-232(helical)-232(stainless)-232(steel)]TJ T* [(wire)-331(spring)-331(\(0.40-mm)-331(wire,)-331(5.0-mm)-331(diameter)-331(spring,)-331(W)74(alter)]TJ T* [(Gogel,)-357(T)92(oledo,)-357(OH\))-357(to)-357(protect)-357(the)-357(tubing)-357(from)-357(manipulation)]TJ T* [(by)-476(the)-476(animal.)-476(This)-476(spring)-476(was)-476(\256xed)-476(on)-476(the)-476(tubing)-476(entry)]TJ T* [(portal)-389(at)-389(the)-389(bottom)-389(end)-389(and)-389(attached)-389(to)-389(the)-389(\257uid)-389(delivery)]TJ T* [(device)-276(at)-276(the)-276(top)-276(end)-276(through)-276(a)-276(lighter)-276(wire)-276(extension)-276(spring)]TJ T* [(\(0.20-mm)-342(wire,)-342(4.0-mm)-342(diameter)-342(spring,)-342(W)74(alter)-342(Gogel\))-342(that)]TJ T* [(was)-200(\257exible)-200(enough)-200(to)-200(allow)-200(maximum)-200(movement)-200(of)-200(the)-200(rat)-200(in)]TJ T* [(the)-360(cage)-360(\(a)-360(standard)-360(polyvinyl)-360(rat)-360(cage,)-360(26.5)]TJ /T1_2 1 Tf 21.366 0 Td (3)Tj /T1_0 1 Tf 1.193 0 Td (20.5)Tj /T1_2 1 Tf 2.306 0 Td (3)Tj /T1_0 1 Tf 1.193 0 Td (16.0)Tj -26.06 -1.111 Td (cm)Tj 5.987 0 0 5.987 60.538 140.389 Tm (3)Tj 8.98 0 0 8.98 63.868 137.645 Tm [(\))-531(with)-531(minimum)-531(tension)-531(on)-531(the)-531(tether)74(.)-531(The)-531(catheters)]TJ -1.704 -1.111 Td [(exiting)-344(from)-344(the)-344(heavier)-344(spring)-344(were)-344(connected)-344(to)-344(the)-344(\257uid-)]TJ 0 -1.111 TD [(delivery)-387(device)-387(by)-387(a)-387(21-gauge,)-387(1.5)-56(\261)-56(2)-387(cm)-387(length)-387(of)-387(stainless)]TJ T* [(steel)-328(tubing.)-328(The)-328(device)-328(provided)-328(a)-328(\257ow)-328(of)-328(saline)-328(containing)]TJ T* [(50)-498(U/ml)-498(of)-498(heparin)-498(at)-498(1)-56(\261)-56(1.5)-498(ml/day)-498(to)-498(maintain)-498(catheter)]TJ 0 -1.111 TD [(patency)111(.)-427(The)-427(\257uid)-427(delivery)-427(device)-427(was)-427(suspended)-427(from)-427(the)]TJ T* [(support)-254(arm)-254(\(Fig.)-254(1)]TJ /T1_1 1 Tf 8.892 0 Td (B)Tj /T1_0 1 Tf 0.722 0 Td [(\))-254(by)-254(a)-254(ball-bearing)-254(\256shing)-254(line)-254(swivel,)-254(so)]TJ -9.614 -1.111 Td [(that)-294(the)-294(device)-294(rotated)-294(freely)111(,)-294(without)-294(twisting)-294(the)-294(tethering)]TJ T* [(spring)-638(or)-638(catheters.)-583(As)-638(a)-638(result,)-638(the)-638(rats)-638(were)-638(allowed)]TJ 29.333 52.667 Td [(essentially)-310(unrestrained)-310(movement.)-310(The)-310(only)-310(required)-310(man-)]TJ T* [(agement)-258(of)-258(the)-258(animal)-258(and)-258(the)-258(device)-258(is)-258(to)-258(re\256ll)-258(the)-258(reservoir)]TJ T* [(bulb)-301(to)-301(a)-301(volume)-301(of)-301(10)-301(ml)-301(about)-301(every)-301(other)-301(day)111(.)-246(All)-301(animals)]TJ T* [(appeared)-221(to)-221(tolerate)-221(the)-221(tethering)-221(device)-221(without)-221(any)-221(discom-)]TJ T* [(fort)-233(or)-233(restriction)-233(of)-233(motion.)]TJ /T1_4 1 Tf 7.982 0 0 7.982 311.987 473.199 Tm [(RESUL)55(TS)]TJ /T1_1 1 Tf 9.978 0 0 9.978 311.987 455.247 Tm [(In)-250(V)62(itro)-250(T)89(ests)-250(of)-250(Flow)-250(and)-250(Pressure)-250(Characteristics)]TJ 1 -1.8 Td [(Constancy)-320(of)-320(\257uid)-320(delivery)55(.)]TJ /T1_0 1 Tf 12.888 0 Td [(Constancy)-320(of)-320(\257uid)-320(deliv-)]TJ -13.888 -1.1 Td [(ery)-405(was)-405(measured)-405(as)-405(a)-405(decrease)-405(in)-405(weight,)-405(using)-405(six)]TJ 0 -1.1 TD [(similar)-301(infusion)-301(devices.)-301(Figure)-301(2)-301(shows)-301(the)-301(decline)-301(in)]TJ T* [(weight)-383(for)-383(each)-383(device)-383(sampled)-383(over)-383(67)-383(h.)-383(The)-383(linear)]TJ T* [(regression)-511(of)-511(weight)-511(versus)-511(time)-511(was)-511(calculated)-511(for)]TJ T* [(each)-285(device;)-285(data)-285(are)-285(shown)-285(in)-285(T)62(able)-285(1.)-285(For)-285(six)-285(devices)]TJ T* [(manufactured)-630(with)-630(resistors)-630(of)-630(similar)-630(length)-630(and)]TJ T* [(diameter)74(,)-467(the)-467(range)-467(of)-467(\257ows)-467(was)-467(from)-467(0.99)-467(to)-467(1.147)]TJ T* [(ml/24)-380(h.)-380(The)-380(square)-380(of)-380(the)-380(Pearson)-380(correlation)-380(coe)10(f)28(f)28(i-)]TJ T* [(cient)-270(for)-270(\257ow)-270(versus)-270(time)-270(was)]TJ /T1_2 1 Tf 13.955 0 Td (.)Tj /T1_0 1 Tf 0.833 0 Td [(0.99)-270(for)-270(all)-270(six)-270(devices,)]TJ -14.788 -1.1 Td [(indicating)-257(that)-257(almost)-257(all)-257(of)-257(the)-257(variability)-257(of)-257(\257ow)-257(with)]TJ T* [(time)-642(\256t)-642(closely)-642(to)-642(a)-642(linear)-642(model)-642(during)-642(67)-642(h)-642(of)]TJ T* (measurement.)Tj /T1_1 1 Tf 1 -1.1 Td [(Reproducibility)-435(of)-435(\257ow)37(.)]TJ /T1_0 1 Tf 11.377 0 Td [(Flow)-435(was)-435(determined)-435(from)]TJ -12.377 -1.1 Td [(\256ve)-607(devices)-607(as)-607(the)-607(decline)-607(in)-607(weight)-607(during)-607(three)]TJ 0 -1.1 TD [(consecutive)-656(24-h)-656(periods.)-656(Reproducibility)-656(was)-656(esti-)]TJ T* [(mated)-403(from)-403(the)-403(percent)-403(coe)10(f)28(f)28(icient)-403(of)-403(variation)-403(\(stan-)]TJ T* [(dard)-391(deviation/mean)]TJ /T1_2 1 Tf 10.06 0 Td (3)Tj /T1_0 1 Tf 1.224 0 Td [(100\))-391(and)-391(ranged)-391(from)-391(1.86)-391(to)]TJ -11.284 -1.1 Td [(8.78%)-233(for)-233(the)-233(\256ve)-233(devices)-233(\(Fig.)-233(3\).)]TJ /T1_1 1 Tf 1 -1.1 Td [(Flow)-547(versus)-547(length)-547(of)-547(resistor)129(.)]TJ /T1_0 1 Tf 15.196 0 Td [(Flow)92(,)-547(measured)-547(in)]TJ -16.196 -1.1 Td [(seven)-428(resistors)-428(varying)-428(in)-428(length)-428(from)-428(30)-428(to)-428(47)-428(mm,)]TJ T* [(declined)-265(by)-265(0.05)-265(ml/24)-265(h)-265(per)-265(millimeter)-265(length.)-265(For)-265(the)]TJ T* [(linear)-301(correlation)-301(between)-301(\257ow)-301(and)-301(length,)-301(the)-301(square)]TJ ET q 48.569 559.159 299.188 169.106 re W n q 0 g 299.9998932 0 0 169.7400055 48.5688019 559.1593018 cm /Im0 Do Q Q BT /T1_0 1 Tf 7.982 0 0 7.982 359.881 648.958 Tm [(Fig.)-331(2.)-556(Six)-331(constant)-331(infusion)-331(devices)-331(showing)-331(decrease)]TJ 0 -1.125 TD [(in)-248(weight)-248(due)-248(to)-248(delivery)-248(of)-248(\257uid)-248(from)-248(devices)-248(over)-248(67)-248(h.)]TJ T* [(Each)-233(symbol)-233(represents)-233(a)-233(single)-233(device.)]TJ 9.978 0 0 9.978 311.987 171.275 Tm [(T)62(able)-250(1.)]TJ /T1_1 1 Tf 4.116 0 Td [(Linear)-250(regression)-250(of)-250(\257ow)-250(during)-250(67)-250(h)-250(for)-250(six)]TJ -4.116 -1.1 Td [(infusion)-250(devices)]TJ ET 0 0 0 1 K 0.499 w 10 M 0 j 0 J []0 d 311.987 154.519 m 563.431 154.519 l 311.987 152.025 m 563.431 152.025 l 311.987 67.941 m 563.428 67.941 l 311.987 128.307 m 563.428 128.307 l S BT /T1_0 1 Tf 6.985 0 0 6.985 323.203 141.528 Tm (Device)Tj 0.74 -1.143 Td (No.)Tj 6.049 1.143 Td [(Flow)92(,)]TJ -0.504 -1.143 Td [(ml/24)-250(h)]TJ /T1_1 1 Tf 8.153 0 Td (R)Tj /T1_0 1 Tf 4.49 0 0 4.49 429.613 135.541 Tm (2)Tj /T1_1 1 Tf 7.187 Tc 6.985 0 0 6.985 477.02 133.546 Tm (FP)Tj /T1_0 1 Tf 0 Tc 7.982 0 0 7.982 333.938 118.08 Tm [(5)-3859(1.036)-3070(0.99777)-2809(2237.01)-3643(8)]TJ /T1_2 1 Tf 24.334 0 Td (3)Tj /T1_0 1 Tf 0.944 0 Td (10)Tj /T1_2 1 Tf 5.488 0 0 5.488 544.598 120.574 Tm (2)Tj /T1_0 1 Tf 0.833 0 Td (8)Tj 7.982 0 0 7.982 333.938 109.1 Tm [(7)-3859(1.068)-3070(0.99778)-2809(2702.06)-2809(3.4)]TJ /T1_2 1 Tf 24.335 0 Td (3)Tj /T1_0 1 Tf 0.944 0 Td (10)Tj /T1_2 1 Tf 5.488 0 0 5.488 544.599 111.594 Tm (2)Tj /T1_0 1 Tf 0.833 0 Td (9)Tj 7.982 0 0 7.982 333.938 100.12 Tm [(9)-3859(1.048)-3070(0.99791)-2809(2862.44)-2809(2.9)]TJ /T1_2 1 Tf 24.335 0 Td (3)Tj /T1_0 1 Tf 0.944 0 Td (10)Tj /T1_2 1 Tf 5.488 0 0 5.488 544.599 102.614 Tm (2)Tj /T1_0 1 Tf 0.833 0 Td (9)Tj 7.982 0 0 7.982 329.502 91.139 Tm [(10)-3859(1.010)-3070(0.99789)-2809(2842.96)-2809(2.9)]TJ /T1_2 1 Tf 24.89 0 Td (3)Tj /T1_0 1 Tf 0.944 0 Td (10)Tj /T1_2 1 Tf 5.488 0 0 5.488 544.599 93.634 Tm (2)Tj /T1_0 1 Tf 0.833 0 Td (9)Tj 7.982 0 0 7.982 329.939 82.159 Tm [(1)55(1)-3859(0.990)-3070(0.99399)-2809(991.662)-2809(6.8)]TJ /T1_2 1 Tf 24.835 0 Td (3)Tj /T1_0 1 Tf 0.944 0 Td (10)Tj /T1_2 1 Tf 5.488 0 0 5.488 544.599 84.654 Tm (2)Tj /T1_0 1 Tf 0.833 0 Td (8)Tj 7.982 0 0 7.982 329.502 73.179 Tm [(12)-3859(1.147)-3070(0.99926)-2837(81)55(12.53)-2837(1.3)]TJ /T1_2 1 Tf 24.89 0 Td (3)Tj /T1_0 1 Tf 0.944 0 Td (10)Tj /T1_1 1 Tf -27.029 -1.933 Td (R)Tj /T1_0 1 Tf 5.488 0 0 5.488 326.328 60.242 Tm (2)Tj 7.982 0 0 7.982 329.38 57.747 Tm [(,)-233(Pearson)-233(correlation)-233(coe)10(f)28(f)28(icient;)]TJ /T1_1 1 Tf 14.863 0 Td (F)Tj /T1_0 1 Tf 0.667 0 Td (,)Tj /T1_1 1 Tf 0.511 0 Td (F)Tj /T1_0 1 Tf 0.899 0 Td (ratio;)Tj /T1_1 1 Tf 2.714 0 Td (P)Tj /T1_0 1 Tf 0.667 0 Td [(,)-233(probability)111(.)]TJ 9.978 0 0 9.978 532.942 744.772 Tm (H2159)Tj 7.982 0 0 7.982 207.629 744.772 Tm [(SELF-POWERED)-366(CONST)55(ANT)-366(INFUSION)-366(DEVICE)]TJ ET 0 G 1 w /GS1 gs 588 18 16 756 re 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m S 0 g /GS1 gs BT /T1_5 1 Tf 0 -9 9 0 593 347.7376 Tm ( on May 20, 2008 )Tj 0 0 1 rg ET /GS1 gs BT /T1_5 1 Tf 0 -9 9 0 593 440.7795 Tm (ajpheart.physiology.org)Tj 0 g ET /GS1 gs BT /T1_5 1 Tf 0 -9 9 0 593 513.8053 Tm (Downloaded from )Tj ET endstream endobj 93 0 obj<> endobj 94 0 obj<> endobj 95 0 obj[93 0 R] endobj 96 0 obj<> endobj 100 0 obj<>stream 0 0 0 1 k 1 i /GS0 gs BT /T1_0 1 Tf 0 Tc 0 Tw 0 Ts 100 Tz 0 Tr 9.978 0 0 9.978 48.569 526.581 Tm [(of)-236(the)-236(Pearson)-236(correlation)-236(coe)10(f)28(f)28(icient)-236(was)-236(0.78,)-236(suggest-)]TJ 0 -1.1 TD [(ing)-224(that)-224(variations)-224(in)-224(length)-224(are)-224(closely)-224(associated)-224(with)]TJ T* [(variations)-233(in)-233(\257ow)92(.)]TJ /T1_1 1 Tf 1 -1.1 Td [(Constancy)-312(of)-312(pressure)-312(head.)]TJ /T1_0 1 Tf 13.204 0 Td [(Simultaneous)-312(measure-)]TJ -14.204 -1.1 Td [(ments)-656(of)-656(pressure)-656(and)-656(volume)-656(were)-656(made)-656(during)]TJ 0 -1.1 TD [(withdrawal)-347(of)-347(water)-347(in)-347(four)-347(bulbs.)-347(T)71(wo)-347(patterns)-347(were)]TJ T* [(seen.)-651(In)-651(two)-651(bulbs,)-651(pressure)-651(declined)-651(slowly)-651(with)]TJ T* [(volume)-230(until)]TJ /T1_2 1 Tf 6.053 0 Td (,)Tj /T1_0 1 Tf 0.833 0 Td [(6)-230(ml)-230(had)-230(been)-230(withdrawn)-230(from)-230(the)-230(bulb.)]TJ 19.513 7.7 Td [(Further)-368(removal)-368(of)-368(water)-368(from)-368(the)-368(bulb)-368(resulted)-368(in)-368(a)]TJ 0 -1.1 TD [(more)-269(rapid)-269(decline)-269(in)-269(pressure)-269(\(Fig.)-269(4,)]TJ /T1_1 1 Tf 17.828 0 Td (A)Tj /T1_0 1 Tf 0.973 0 Td (and)Tj /T1_1 1 Tf 2.01 0 Td (B)Tj /T1_0 1 Tf 0.722 0 Td [(\).)-269(In)-269(the)]TJ -21.534 -1.1 Td [(other)-294(two)-294(bulbs,)-294(pressure)-294(increased)-294(slightly)-294(as)-294(volume)]TJ T* [(decreased,)-347(until)-347(about)-347(6)-347(ml)-347(had)-347(been)-347(withdrawn,)-347(and)]TJ T* [(then)-650(pressure)-650(declined)-650(rapidly)-650(\(Fig.)-650(5,)]TJ /T1_1 1 Tf 20.123 0 Td (A)Tj /T1_0 1 Tf 1.354 0 Td (and)Tj /T1_1 1 Tf 2.391 0 Td (B)Tj /T1_0 1 Tf 0.722 0 Td (\).)Tj -24.59 -1.1 Td [(Similar)-346(patterns)-346(were)-346(seen)-346(for)-346(all)-346(of)-346(the)-346(trials)-346(for)-346(the)]TJ 0 -1.1 TD [(bulbs)-356(that)-356(showed)-356(an)-356(initial)-356(increase)-356(in)-356(pressure)-356(and)]TJ T* [(for)-424(those)-424(that)-424(showed)-424(a)-424(decrease.)-424(Considering)-424(all)-424(17)]TJ ET q 264.093 552.155 299.188 176.111 re W n q 0 g 299.9998932 0 0 176.2200012 264.0926056 552.1549072 cm /Im0 Do Q Q BT /T1_0 1 Tf 7.982 0 0 7.982 48.569 646.464 Tm [(Fig.)-430(3.)-556(Three)-430(consecutive)-430(measurements)-430(of)-430(\257ow)-430(from)]TJ 0 -1.125 TD [(each)-562(of)-562(\256ve)-562(devices,)-562(showing)-562(reproducibility)-562(of)-562(\257ow)]TJ T* [(measurement.)-233(CV)129(,)-233(coe)10(f)28(f)28(icient)-233(of)-233(variation.)]TJ ET q 264.093 59.689 299.188 371.234 re W n q 0 g 299.9998932 0 0 371.7899017 264.0926056 59.6891022 cm /Im1 Do Q Q BT /T1_0 1 Tf 7.982 0 0 7.982 48.569 250.339 Tm [(Fig.)-223(4.)-556(Simultaneous)-223(measurement)-223(of)-223(volume)-223(and)-223(pres-)]TJ T* [(sure)-266(in)-266(bulb,)-266(showing)-266(two)-266(bulbs)-266(\()]TJ /T1_1 1 Tf 15.168 0 Td (A)Tj /T1_0 1 Tf 0.704 0 Td (:)Tj /T1_1 1 Tf 0.544 0 Td [(bulb)-266(1)]TJ /T1_0 1 Tf 2.877 0 Td (;)Tj /T1_1 1 Tf 0.544 0 Td (B)Tj /T1_0 1 Tf 0.722 0 Td (:)Tj /T1_1 1 Tf 0.544 0 Td [(bulb)-266(2)]TJ /T1_0 1 Tf 0.266 Tc 2.877 0 Td [(\)i)266(n)]TJ 0 Tc -23.98 -1.125 Td [(which)-233(pressure)-233(decreased)-233(with)-233(decreasing)-233(volume.)]TJ 9.978 0 0 9.978 48.569 744.772 Tm (H2160)Tj 7.982 0 0 7.982 207.629 744.772 Tm [(SELF-POWERED)-366(CONST)55(ANT)-366(INFUSION)-366(DEVICE)]TJ ET 0 G 1 w 10 M 0 j 0 J []0 d /GS1 gs 588 18 16 756 re 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m S 0 g /GS1 gs BT /T1_3 1 Tf 0 -9 9 0 593 347.7376 Tm ( on May 20, 2008 )Tj 0 0 1 rg ET /GS1 gs BT /T1_3 1 Tf 0 -9 9 0 593 440.7795 Tm (ajpheart.physiology.org)Tj 0 g ET /GS1 gs BT /T1_3 1 Tf 0 -9 9 0 593 513.8053 Tm (Downloaded from )Tj ET endstream endobj 101 0 obj<> endobj 102 0 obj<> endobj 103 0 obj[101 0 R] endobj 104 0 obj<> endobj 108 0 obj<>stream 0 0 0 1 k 1 i /GS0 gs BT /T1_0 1 Tf 0 Tc 0 Tw 0 Ts 100 Tz 0 Tr 9.978 0 0 9.978 48.569 338.224 Tm [(trials)-376(in)-376(the)-376(four)-376(bulbs,)-376(during)-376(the)-376(withdrawal)-376(of)-376(the)]TJ 0 -1.1 TD [(\256rst)-556(milliliter)-556(of)-556(water)-556(from)-556(the)-556(bulb)-556(the)-556(range)-556(of)]TJ T* [(change)-233(in)-233(pressure)-233(was)]TJ /T1_1 1 Tf 10.912 0 Td (1)Tj /T1_0 1 Tf 0.833 0 Td [(1.73)-233(to)]TJ /T1_1 1 Tf 3.301 0 Td (2)Tj /T1_0 1 Tf 0.833 0 Td [(6.65)-233(mmHg.)]TJ /T1_2 1 Tf -15.879 -1.8 Td [(In)-250(V)62(ivo)-250(Experiments:)-250(Use)-250(of)-250(Flow)-250(Device)-250(to)-250(Maintain)]TJ 0 -1.1 TD [(Catheter)-250(Patency)]TJ /T1_0 1 Tf 1 -1.8 Td [(The)-537(\257uid)-537(delivery)-537(devices)-537(were)-537(used)-537(to)-537(maintain)]TJ -1 -1.1 Td [(patent)-360(arterial)-360(or)-360(venous)-360(catheters)-360(in)-360(100)-360(chronically)]TJ T* [(instrumented)-333(rats.)-334(In)-333(vivo,)-334(\257uid)-333(delivery)-333(devices)-333(with)]TJ T* [(resistors)-489(of)-489(3.2)-489(cm)-489(in)-489(length)-489(produced)-489(a)-489(\257ow)-489(of)]TJ /T1_3 1 Tf 23.813 0 Td (,)Tj /T1_0 1 Tf 0.833 0 Td (1)Tj -24.646 -1.1 Td [(ml/day)-253(in)-253(the)-253(arterial)-253(catheters)-253(and)]TJ /T1_3 1 Tf 16.649 0 Td (,)Tj /T1_0 1 Tf 0.833 0 Td [(1.5)-253(ml/day)-253(in)-253(the)]TJ -17.482 -1.1 Td [(venous)-401(catheters.)-401(Both)-401(arterial)-401(and)-401(venous)-401(catheters)]TJ T* [(were)-215(kept)-215(patent)-215(on)-215(average)-215(for)-215(as)-215(long)-215(as)-215(3)-215(wk)-215(without)]TJ T* [(any)-291(need)-291(for)-291(additional)-291(\257ushing.)-291(No)-291(experiments)-291(were)]TJ T* [(terminated)-233(because)-233(of)-233(catheter)-233(failure.)]TJ 1 -1.1 Td [(All)-291(animals)-291(were)-291(maintained)-291(in)-291(good)-291(health)-291(with)-291(an)]TJ -1 -1.1 Td [(average)-539(daily)-539(body)-539(weight)-539(gain)-539(of)-539(4.6)-539(g.)-539(The)-539(daily)]TJ 0 -1.1 TD [(consumption)-218(of)-218(food)-218(and)-218(water)-218(was)-218(in)-218(the)-218(normal)-218(range)]TJ T* [(for)-233(rats)-233(of)-233(this)-233(age)-233(\(23.2)]TJ /T1_1 1 Tf 11.009 0 Td (6)Tj /T1_0 1 Tf 1.066 0 Td [(0.7)-233(g)-233(and)-233(26.3)]TJ /T1_1 1 Tf 6.546 0 Td (6)Tj /T1_0 1 Tf 1.066 0 Td [(0.7)-233(ml\).)]TJ /T1_4 1 Tf 7.982 0 0 7.982 48.569 119.699 Tm (DISCUSSION)Tj /T1_0 1 Tf 9.978 0 0 9.978 58.547 101.747 Tm [(Chronic)-294(intravascular)-294(infusion)-294(in)-294(small)-294(animals)-294(is)-294(a)]TJ -1 -1.1 Td [(useful)-611(experimental)-611(technique)-611(in)-611(physiological)-611(and)]TJ 0 -1.1 TD [(pharmacological)-256(studies.)-256(The)-256(infusion)-256(device)-256(described)]TJ T* [(here)-222(provides)-222(an)-222(e)10(f)28(fective)-222(long-term)-222(access)-222(for)-222(adminis-)]TJ T* [(tration)-441(of)-441(experimental)-441(agents)-441(with)-441(minimal)-441(volume)]TJ 26.4 28.1 Td [(loading,)-555(repeated)-555(hemodynamic)-555(measurements,)-555(and)]TJ 0 -1.13 TD [(blood)-450(sampling)-450(in)-450(small,)-450(unrestrained)-450(animals.)-450(This)]TJ T* [(device)-459(is)-459(unique)-459(in)-459(that)-459(the)-459(delivery)-459(of)-459(\257uid)-459(is)-459(self-)]TJ T* [(powered)-370(by)-370(the)-370(elasticity)-370(of)-370(the)-370(rubber)-370(bulb.)-370(Because)]TJ T* [(the)-281(device)-281(is)-281(self-powered)-281(and)-281(thus)-281(self-contained,)-281(and)]TJ T* [(because)-270(it)-270(weighs)-270(only)]TJ /T1_3 1 Tf 10.581 0 Td (,)Tj /T1_0 1 Tf 0.833 0 Td [(20)-270(g)-270(\(including)-270(10)-270(g)-270(of)-270(saline\),)]TJ -11.414 -1.13 Td [(it)-465(can)-465(be)-465(suspended)-465(by)-465(a)-465(simple)-465(\256shing)-465(line)-465(swivel.)]TJ T* [(Consequently)111(,)-304(the)-304(device)-304(rotates)-304(freely)-304(with)-304(the)-304(move-)]TJ T* [(ment)-370(of)-370(the)-370(animal.)-370(Furthermore,)-370(the)-370(light)-370(extension)]TJ T* [(spring)-374(attaching)-374(the)-374(device)-374(to)-374(the)-374(catheter)18(-protecting)]TJ T* [(spring)-222(allows)-222(lateral)-222(movements)-222(with)-222(minimal)-222(tension.)]TJ T* [(As)-376(a)-376(result,)-376(movement)-376(of)-376(the)-376(animal)-376(within)-376(its)-376(home)]TJ 0 -1.12 TD [(cage)-387(is)-387(essentially)-387(unrestricted.)-387(The)-387(device)-387(is)-387(easy)-387(to)]TJ T* [(manufacture,)-206(and)-206(all)-206(the)-206(required)-206(materials)-206(are)-206(readily)]TJ T* [(available.)-460(The)-460(approximate)-460(cost)-460(for)-460(the)-460(materials)-460(to)]TJ T* [(create)-479(one)-479(\257ow)-479(device)-479(is)-479(less)-479(than)-479($10.)-479(By)-479(creating)]TJ T* [(continuous)-326(\257uid)-326(infusion)-326(without)-326(interrupting)-326(normal)]TJ T* [(activity)-308(of)-308(the)-308(animal,)-308(this)-308(device)-308(eliminates)-308(the)-308(prob-)]TJ 0 -1.12 TD [(lems)-424(that)-424(often)-424(occur)-424(in)-424(chronic)-424(cannulations)-424(with)-424(a)]TJ T* [(pump)-653(mounted)-653(outside)-653(of)-653(the)-653(animal)-653(cage)-653(and)-653(a)]TJ T* [(commercial)-651(swivel.)-651(By)-651(using)-651(this)-651(device)-651(to)-651(infuse)]TJ T* [(heparinized)-230(saline)-230(at)-230(1.0)-56(\261)-56(1.5)-230(ml/day)-230(in)-230(over)-230(100)-230(rats)-230(in)]TJ T* [(our)-234(laboratory)111(,)-234(we)-233(have)-233(observed)-233(that)-234(both)-233(arterial)-234(and)]TJ T* [(venous)-259(catheters)-259(could)-259(be)-259(kept)-259(patent)-259(for)-259(a)-259(period)-259(of)-259(at)]TJ T* [(least)-220(3)-220(wk)-220(without)-220(daily)-220(\257ushing.)-220(In)-220(one)-220(study)-220(from)-220(our)]TJ T* [(laboratory)-570(\(9\))-570(Dahl)-570(salt-sensitive)-570(and)-570(salt-resistant)]TJ ET q 48.569 368.039 303.191 360.227 re W n q 0 g 303.1199036 0 0 360.809906 48.5688019 368.039093 cm /Im0 Do Q Q BT /T1_0 1 Tf 7.982 0 0 7.982 362.874 558.76 Tm [(Fig.)-655(5.)-556(Simultaneous)-655(measurement)-655(of)-655(volume)-655(and)]TJ 0 -1.125 TD [(pressure)-265(in)-264(bulb,)-265(showing)-265(two)-265(bulbs)-265(\()]TJ /T1_2 1 Tf 17.143 0 Td (A)Tj /T1_0 1 Tf 0.704 0 Td (:)Tj /T1_2 1 Tf 0.543 0 Td [(bulb)-265(3)]TJ /T1_0 1 Tf 2.876 0 Td (;)Tj /T1_2 1 Tf 0.543 0 Td (B)Tj /T1_0 1 Tf 0.722 0 Td (:)Tj /T1_2 1 Tf 0.543 0 Td (bulb)Tj -23.073 -1.125 Td (4)Tj /T1_0 1 Tf 0.556 0 Td [(\))-207(in)-207(which)-207(pressure)-207(initially)-207(increased)-207(with)-207(decreasing)]TJ -0.556 -1.125 Td (volume.)Tj 9.978 0 0 9.978 532.942 744.772 Tm (H2161)Tj 7.982 0 0 7.982 207.629 744.772 Tm [(SELF-POWERED)-366(CONST)55(ANT)-366(INFUSION)-366(DEVICE)]TJ ET 0 G 1 w 10 M 0 j 0 J []0 d /GS1 gs 588 18 16 756 re 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m S 0 g /GS1 gs BT /T1_5 1 Tf 0 -9 9 0 593 347.7376 Tm ( on May 20, 2008 )Tj 0 0 1 rg ET /GS1 gs BT /T1_5 1 Tf 0 -9 9 0 593 440.7795 Tm (ajpheart.physiology.org)Tj 0 g ET /GS1 gs BT /T1_5 1 Tf 0 -9 9 0 593 513.8053 Tm (Downloaded from )Tj ET endstream endobj 109 0 obj<> endobj 110 0 obj<> endobj 111 0 obj[109 0 R] endobj 112 0 obj<> endobj 116 0 obj<>stream 0 0 0 1 k 1 i /GS0 gs BT /T1_0 1 Tf 0 Tc 0 Tw 0 Ts 100 Tz 0 Tr 9.978 0 0 9.978 48.569 720.825 Tm [(inbred)-485(rats)-485(were)-485(successfully)-485(instrumented)-485(with)-485(ca-)]TJ 0 -1.1 TD [(rotid)-516(arterial)-516(and)-516(jugular)-516(venous)-516(catheters)-516(that)-516(re-)]TJ T* [(mained)-212(patent)-212(for)-212(15)-212(days.)-212(The)-212(infusion)-212(device)-212(was)-212(also)]TJ T* [(used)-336(to)-336(obtain)-336(blood)-336(samples)-336(for)-336(serial)-336(measurements)]TJ T* [(of)-243(hematocrit)-243(and)-243(plasma)-243(volume)-243(\(by)-243(dilution)-243(of)-243(Evan')37(s)]TJ 0 -1.1 TD [(blue\).)-234(In)-234(a)-234(second)-234(study)-234(from)-234(our)-234(laboratory)111(,)-234(DiPaola)-234(et)]TJ T* [(al.)-512(\(4\))-512(used)-512(the)-512(infusion)-512(device)-512(for)-512(measurement)-512(of)]TJ T* [(arterial)-476(pressure)-476(and)-476(to)-476(observe)-476(the)-476(acute)-476(e)10(f)28(fects)-476(of)]TJ T* [(captopril)-620(and)-620(saralasin)-620(on)-620(arterial)-620(blood)-620(pressure.)]TJ T* [(These)-298(two)-298(studies)-298(illustrate)-298(the)-298(utility)-298(of)-298(the)-298(device)-298(in)]TJ -0.007 Tc T* [(maintaining)-192(patent)-192(vascular)-192(catheters,)-192(measuring)-192(blood)]TJ 0 Tc T* [(pressures,)-215(and)-215(administering)-215(experimental)-215(agents)-215(dur)18(-)]TJ T* [(ing)-233(various)-233(experimental)-233(maneuvers.)]TJ 1 -1.1 Td [(Infusion)-203(of)-203(only)-203(1)-203(to)-203(1.5)-203(ml/day)-203(allows)-203(maintenance)-203(of)]TJ -1 -1.1 Td [(catheter)-585(patency)-585(or)-585(administration)-585(of)-585(experimental)]TJ T* [(agents)-217(with)-217(minimal)-217(\257uid)-217(volume)-217(expansion)-217(and)-217(there-)]TJ 0 -1.1 TD [(fore)-261(presumably)-261(minimal)-261(e)10(f)28(fects)-261(on)-261(cardiovascular)-261(and)]TJ T* [(renal)-233(function.)]TJ 1 -1.1 Td [(Adjusting)-262(the)-262(length)-262(and)-262(inner)-262(diameter)-262(of)-262(the)-262(silica)]TJ -1 -1.1 Td [(capillary)-304(tubing)-304(in)-304(the)-304(resistor)-304(achieves)-304(variable)-304(rates)]TJ T* [(of)-372(\257uid)-372(delivery)-372(to)-372(suit)-372(the)-372(needs)-372(of)-372(di)10(f)28(ferent)-372(studies.)]TJ T* [(Given)-307(our)-307(observation)-307(that)-307(the)-307(rate)-307(of)-307(\257uid)-307(delivery)-307(is)]TJ T* [(linear)-313(and)-313(nearly)-313(constant)-313(over)-313(67)-313(h)-313(\(Fig.)-313(2)-313(and)-313(T)62(able)]TJ T* [(1\),)-579(the)-579(device)-579(may)-579(be)-579(used)-579(to)-579(infuse)-579(experimental)]TJ T* [(agents)-233(at)-233(known)-233(rates)-233(for)-233(several)-233(days.)]TJ 1 -1.1 Td [(Constancy)-461(of)-461(\257ow)-461(is)-461(a)-461(result)-461(of)-461(the)-461(use)-461(of)-461(a)-461(\256xed)]TJ -1 -1.1 Td [(resistor)-368(and)-368(the)-368(minimal)-368(changes)-368(in)-368(driving)-368(pressure)]TJ T* [(observed)-533(during)-533(the)-533(simultaneous)-533(measurements)-534(of)]TJ T* [(pressure)-381(and)-381(volume)-381(in)-381(the)-381(pressure)-381(bulbs.)-326(Although)]TJ T* [(two)-509(patterns)-509(of)-509(change)-509(in)-509(pressure)-509(\(increase)-509(or)-509(de-)]TJ 0 -1.1 TD [(crease\))-375(with)-375(declining)-375(volume)-375(were)-375(noted)-375(in)-375(di)10(f)28(ferent)]TJ 0 -1.1 TD [(bulbs,)-558(the)-558(range)-558(of)-558(changes)-558(in)-558(pressure)-558(was)-558(small)]TJ T* (\()Tj /T1_1 1 Tf 0.333 0 Td (1)Tj /T1_0 1 Tf 0.833 0 Td [(1.73)-232(to)]TJ /T1_1 1 Tf 3.298 0 Td (2)Tj /T1_0 1 Tf 0.833 0 Td [(6.65)-232(mmHg\))-232(during)-232(delivery)-232(of)-232(1)-232(ml)-232(of)-232(\257uid.)]TJ -5.297 -1.1 Td [(Delivery)-557(of)-557(1)-557(ml)-557(of)-557(\257uid)-557(over)-557(24)-557(h)-557(is)-557(su)10(f)28(f)28(icient)-557(to)]TJ T* [(maintain)-233(catheter)-233(patency)111(.)]TJ 1 -1.1 Td [(W)55(e)-239(have)-239(no)-239(data)-239(to)-239(account)-239(for)-239(the)-239(small)-239(increases)-239(or)]TJ -1 -1.1 Td [(decreases)-326(in)-326(pressure)-326(that)-326(occurred)-326(during)-326(delivery)-326(of)]TJ T* [(\257uid)-537(from)-537(the)-537(bulb)-537(but)-537(would)-537(speculate)-537(that)-537(these)]TJ T* [(pressure)-570(patterns)-570(are)-570(a)-570(result)-570(of)-570(variations)-570(in)-570(the)]TJ T* [(characteristics)-647(of)-647(di)10(f)28(ferent)-647(pressure)-647(bulbs.)-647(For)-647(ex-)]TJ T* [(ample,)-618(from)-618(the)-618(law)-618(of)-618(Laplace)-618(\(see)]TJ /T1_2 1 Tf 18.992 0 Td [(Constancy)-618(of)]TJ -18.992 -1.1 Td [(pressure)-344(head)]TJ /T1_0 1 Tf 6.432 0 Td [(\),)-344(if)-344(the)-344(radius)-344(of)-344(the)-344(bulb)-344(declined)-344(with)]TJ -6.432 -1.1 Td [(decreasing)-453(volume)-453(more)-453(quickly)-453(than)-453(the)-453(product)-453(of)]TJ 26.4 46.2 Td [(wall)-587(stress)-587(and)-587(wall)-587(thickness,)-587(pressure)-587(would)-587(in-)]TJ 0 -1.1 TD [(crease.)-630(These)-630(changes)-630(presumably)-630(account)-630(for)-630(the)]TJ T* [(observation)-402(that)-402(in)-402(two)-402(bulbs)-402(pressure)-402(increased)-402(ini-)]TJ T* [(tially)-233(with)-233(\257uid)-233(delivery)111(.)]TJ 1 -1.1 Td [(In)-386(summary)111(,)-386(the)-386(self-powered)-386(catheter)-386(infusion)-386(de-)]TJ -1 -1.1 Td [(vice)-399(we)-399(developed)-399(o)10(f)28(fers)-399(advantages)-399(of)-399(simplicity)111(,)-399(de-)]TJ 0 -1.1 TD [(pendability)111(,)-574(capacity)-574(for)-574(quantitative)-574(adjustment)-574(of)]TJ -0.003 Tc T* [(continuous)-197(\257ow)-197(over)-197(extended)-197(periods,)-197(ease)-197(of)-197(manufac-)]TJ 0 Tc T* [(ture,)-222(minimal)-222(expense,)-222(and)-222(unrestrained)-222(activity)-222(of)-222(the)]TJ T* (animal.)Tj 7.982 0 0 7.982 321.965 607.561 Tm [(Address)-567(for)-567(reprint)-567(requests)-567(and)-567(other)-567(correspondence:)-567(P)129(.)-567(H.)]TJ -1.25 -1.125 Td [(Brand,)-383(Physiology)-383(&)-383(Molecular)-383(Medicine,)-383(Medical)-383(College)-383(of)-383(Ohio,)]TJ 0.014 Tc 0 -1.125 TD [(3035)-624(Arlington)-624(A)74(ve.,)-679(T)92(oledo,)-679(OH)-679(43614-5804)-679(\(E-mail:)-679(pbrand)]TJ 0 Tc T* [(@)-233(mco.edu\).)]TJ 0 -1.625 TD [(Received)-222(1)-222(November)-222(1999;)-222(accepted)-222(in)-222(\256nal)-222(form)-222(20)-222(December)-222(1999.)]TJ /T1_3 1 Tf 0 -2.064 TD (REFERENCES)Tj /T1_0 1 Tf 0.556 -1.625 Td (1.)Tj /T1_3 1 Tf 1.39 0 Td [(Ardill)-480(BL)-462(and)-480(Fentem)-480(PH.)]TJ /T1_0 1 Tf 14.715 0 Td [(A)-425(simple)-480(device)-480(for)-480(continuous)]TJ -0.007 Tc -14.715 -1.125 Td [(\257ushing)-193(of)-193(catheter)-193(systems)-193(during)-193(intra-arterial)-193(pressure)-193(record-)]TJ 0 Tc 0 -1.125 TD (ing.)Tj /T1_2 1 Tf 1.973 0 Td [(Cardiovasc)-233(Res)]TJ /T1_0 1 Tf 7.258 0 Td [(1:)-233(294)-56(\261)-56(296,)-233(1967.)]TJ -10.621 -1.125 Td (2.)Tj /T1_3 1 Tf 1.39 0 Td [(Berne)-426(RM)-426(and)-426(Levy)-426(MN.)]TJ /T1_2 1 Tf 13.628 0 Td (Physiology)Tj /T1_0 1 Tf 5.351 0 Td [(St.)-426(Louis,)-426(MO:)-426(Mosby)111(,)]TJ -18.979 -1.125 Td [(1998,)-233(p.)-233(431.)]TJ -1.39 -1.125 Td (3.)Tj /T1_3 1 Tf 1.39 0 Td [(Cox)-406(CE)-406(and)-406(Beazley)-406(RM.)]TJ /T1_0 1 Tf 13.639 0 Td [(Chronic)-406(venous)-406(catheterization:)-406(a)]TJ -13.639 -1.125 Td [(technique)-392(for)-392(implanting)-392(and)-392(maintaining)-392(venous)-392(catheters)-392(in)]TJ T* (rats.)Tj /T1_2 1 Tf 2.362 0 Td [(J)-233(Surg)-233(Res)]TJ /T1_0 1 Tf 5.196 0 Td [(18:)-233(607)-56(\261)-56(610,)-233(1975.)]TJ -8.948 -1.125 Td (4.)Tj /T1_3 1 Tf 1.39 0 Td [(DiPaola)-204(NR,)-204(Rapp)-204(JP)129(,)-204(Brand)-204(PH,)-204(Beierwaltes)-204(WH,)-204(Metting)]TJ T* [(PJ,)-206(and)-206(Britton)-206(SL.)]TJ /T1_0 1 Tf 9.954 0 Td [(Evaluation)-206(of)-206(the)-206(renin)-206(angiotensin)-206(system)]TJ -9.954 -1.125 Td [(in)-208(a)-208(congenic)-208(renin)-208(Dahl)-208(salt-sensitive)-208(rat.)]TJ /T1_2 1 Tf 19.456 0 Td [(Genes)-208(and)-208(Function)]TJ /T1_0 1 Tf 9.269 0 Td (1:)Tj -28.725 -1.125 Td [(215)-56(\261)-56(226,)-233(1997.)]TJ -1.39 -1.125 Td (5.)Tj /T1_3 1 Tf 1.39 0 Td [(Gibbs)-419(SR.)]TJ /T1_0 1 Tf 5.597 0 Td [(A)-364(simple)-419(disposable)-419(continuous-infusion)-419(swivel)-419(for)]TJ -5.597 -1.125 Td [(unrestrained)-579(small)-580(animals.)]TJ /T1_2 1 Tf 14.276 0 Td [(J)-579(Appl)-580(Physiol)]TJ /T1_0 1 Tf 7.922 0 Td [(70:)-580(2764)-56(\261)-56(2765,)]TJ -22.198 -1.125 Td (1991.)Tj -1.39 -1.125 Td (6.)Tj /T1_3 1 Tf 1.39 0 Td [(Goldstein)-456(JM.)]TJ /T1_0 1 Tf 7.837 0 Td [(Intravenous)-456(infusion)-456(system)-456(for)-456(chronic)-456(drug)]TJ -7.837 -1.125 Td [(administration)-285(in)-285(unrestrained)-285(rats.)]TJ /T1_2 1 Tf 17.141 0 Td [(Pharmacol)-285(Biochem)-285(Behav)]TJ /T1_0 1 Tf -17.141 -1.125 Td [(4:)-233(613)-56(\261)-56(615,)-233(1976.)]TJ -1.39 -1.125 Td (7.)Tj /T1_3 1 Tf 1.39 0 Td [(Hodge)-507(DE)-507(and)-507(Shalev)-507(M.)]TJ /T1_0 1 Tf 14.18 0 Td [(Dual)-507(cannulation:)-507(a)-507(method)-507(for)]TJ -14.18 -1.125 Td [(continuous)-258(intravenous)-258(infusion)-258(and)-258(repeated)-258(blood)-258(sampling)-258(in)]TJ T* [(unrestrained)-233(mice.)]TJ /T1_2 1 Tf 8.908 0 Td [(Lab)-233(Anim)-233(Sci)]TJ /T1_0 1 Tf 6.475 0 Td [(42:)-233(320)-56(\261)-56(322,)-233(1992.)]TJ -16.773 -1.125 Td (8.)Tj /T1_3 1 Tf 1.39 0 Td [(Matsumura)-311(H,)-311(Kinoshita)-311(G,)-311(Satoh)-311(S,)-311(Osaka)-311(T)92(,)-311(and)-311(Hayai-)]TJ T* [(shi)-603(O.)]TJ /T1_0 1 Tf 3.872 0 Td [(A)-549(novel)-603(apparatus)-603(that)-603(permits)-603(multiple)-603(routes)-603(for)]TJ -3.872 -1.125 Td [(infusions)-279(and)-279(body-\257uid)-279(collections)-279(in)-279(a)-279(freely-moving)-279(animal.)]TJ /T1_2 1 Tf 28.95 0 Td (J)Tj -28.95 -1.125 Td [(Neurosci)-233(Methods)]TJ /T1_0 1 Tf 8.405 0 Td [(57:)-233(145)-56(\261)-56(149,)-233(1995.)]TJ -9.795 -1.125 Td (9.)Tj /T1_3 1 Tf 1.39 0 Td [(Qi)-202(N,)-202(Rapp)-202(JP)129(,)-202(Brand)-202(PH,)-202(Metting)-202(PJ,)-202(and)-202(Britton)-202(SL.)]TJ /T1_0 1 Tf 27.224 0 Td (Body)Tj -27.224 -1.125 Td [(\257uid)-306(expansion)-306(is)-306(not)-306(essential)-306(for)-306(salt-induced)-306(hypertension)-306(in)]TJ T* [(SS/Jr)-397(rats.)]TJ /T1_2 1 Tf 5.462 0 Td [(Am)-397(J)-397(Physiol)-397(Regulatory)-397(Integrative)-397(Comp)-397(Physiol)]TJ /T1_0 1 Tf -5.462 -1.125 Td [(277:)-233(R1392)-56(\261)-56(R1400,)-233(1999.)]TJ -1.945 -1.125 Td (10.)Tj /T1_3 1 Tf 1.946 0 Td [(T)62(sui)-240(BCH,)-240(Mosher)-240(SJ,)-240(and)-203(Y)111(eung)-240(KF)111(.)]TJ /T1_0 1 Tf 18.857 0 Td [(A)-185(reliable)-240(technique)-240(for)]TJ -18.858 -1.125 Td [(chronic)-339(carotid)-339(arterial)-339(catheterization)-339(in)-339(the)-339(rat.)]TJ /T1_2 1 Tf 23.575 0 Td [(J)-339(Pharmacol)]TJ -23.575 -1.125 Td (Methods)Tj /T1_0 1 Tf 4.138 0 Td [(25:)-233(343)-56(\261)-56(352,)-233(1991.)]TJ 9.978 0 0 9.978 48.569 744.772 Tm (H2162)Tj 7.982 0 0 7.982 207.629 744.772 Tm [(SELF-POWERED)-366(CONST)55(ANT)-366(INFUSION)-366(DEVICE)]TJ ET 0 G 1 w 10 M 0 j 0 J []0 d /GS1 gs 588 18 16 756 re 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m 0 0 m S 0 g /GS1 gs BT /T1_4 1 Tf 0 -9 9 0 593 347.7376 Tm ( on May 20, 2008 )Tj 0 0 1 rg ET /GS1 gs BT /T1_4 1 Tf 0 -9 9 0 593 440.7795 Tm (ajpheart.physiology.org)Tj 0 g ET /GS1 gs BT /T1_4 1 Tf 0 -9 9 0 593 513.8053 Tm (Downloaded from )Tj ET endstream endobj 117 0 obj<>stream 2000-05-31T14:02:03Z Parlance Publisher 5.0/(Xyvision Postscript Formatter) 3.0 3 2008-05-20T06:39:42Z Acrobat Distiller 3.01 for Windows application/pdf AHEART June 47/6 uuid:a330228e-1dd1-11b2-0a00-10a1100a3c59 uuid:a3302298-1dd1-11b2-0a00-0000000048c5 endstream endobj xref 0 118 0000000002 00000 f 0000000016 00000 n 0000000003 00001 f 0000000014 00001 f 0000000240 00000 n 0000001281 00000 n 0000002330 00000 n 0000003377 00000 n 0000004424 00000 n 0000005355 00000 n 0000005418 00000 n 0000005533 00000 n 0000034607 00000 n 0000062829 00000 n 0000000015 00001 f 0000000020 00001 f 0000063090 00000 n 0000064099 00000 n 0000065109 00000 n 0000067305 00000 n 0000000021 00001 f 0000000025 00001 f 0000067555 00000 n 0000078467 00000 n 0000082622 00000 n 0000000026 00001 f 0000000029 00001 f 0000082861 00000 n 0000086939 00000 n 0000000030 00001 f 0000000032 00001 f 0000087192 00000 n 0000000033 00001 f 0000000034 00001 f 0000000073 00000 f 0000087403 00000 n 0000087579 00000 n 0000087754 00000 n 0000097328 00000 n 0000097505 00000 n 0000104992 00000 n 0000105179 00000 n 0000105364 00000 n 0000105547 00000 n 0000105725 00000 n 0000114189 00000 n 0000114922 00000 n 0000115655 00000 n 0000115718 00000 n 0000115938 00000 n 0000116144 00000 n 0000116242 00000 n 0000116338 00000 n 0000116397 00000 n 0000116496 00000 n 0000116577 00000 n 0000116675 00000 n 0000116766 00000 n 0000116864 00000 n 0000116940 00000 n 0000117045 00000 n 0000117100 00000 n 0000117201 00000 n 0000117263 00000 n 0000117353 00000 n 0000117444 00000 n 0000117533 00000 n 0000117628 00000 n 0000117716 00000 n 0000120852 00000 n 0000120953 00000 n 0000121015 00000 n 0000121039 00000 n 0000000074 00001 f 0000000075 00001 f 0000000081 00001 f 0000121088 00000 n 0000133796 00000 n 0000133897 00000 n 0000133959 00000 n 0000133983 00000 n 0000000082 00001 f 0000000083 00001 f 0000000089 00001 f 0000134032 00000 n 0000145723 00000 n 0000145824 00000 n 0000145886 00000 n 0000145910 00000 n 0000000090 00001 f 0000000091 00001 f 0000000097 00001 f 0000145959 00000 n 0000158726 00000 n 0000158827 00000 n 0000158889 00000 n 0000158913 00000 n 0000000098 00001 f 0000000099 00001 f 0000000105 00001 f 0000158962 00000 n 0000162714 00000 n 0000162817 00000 n 0000162880 00000 n 0000162906 00000 n 0000000106 00001 f 0000000107 00001 f 0000000113 00001 f 0000162956 00000 n 0000169856 00000 n 0000169959 00000 n 0000170022 00000 n 0000170048 00000 n 0000000114 00001 f 0000000115 00001 f 0000000000 00001 f 0000170098 00000 n 0000181258 00000 n trailer <]>> startxref 182711 %%EOF