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Department of Physiology and Pharmacology

Ritu Chakravarti, Ph.D.

Ritu Chakravarti, Ph.D.

Associate Professor
Office: 419.383.4468
Laboratory: 419.383.4212
Fax: 419.383.2871
贰尘补颈濒:听Ritu.Chakravarti@utoledo.edu听
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Recent Honors

  • Presidential Award for Excellence in Creative & Scholarship Activity 2024, The University of Toledo.
  • Innovator Award, Influential 蘑菇视频 Women, 2023, Catherine S. Eberly Center for Women, The University of Toledo.听
  • Dean鈥檚 Award for New Investigator Research Excellence 2022, The University of Toledo College of Medicine and Life Sciences.听
  • Presidential Award for Excellence in Creative & Scholarship Activity 2022, The University of Toledo.
  • Nominated for Best Advisor Award 2022, The University of Toledo.

Funding Sources

NIH, ODH-CDC, AHA, Vasculitis Foundation, Medical Research Society, 蘑菇视频 Research Foundation

Research interest

Our research focuses on understanding how 14-3-3z regulates the immune system and its role in various autoimmune diseases.

A) Vaccine for Rheumatoid Arthritis

Rheumatoid arthritis, vasculitis, and lupus are some examples of autoimmune diseases. Few autoantigens are known to associate with these diseases; however, their role in the pathogenesis remains mostly unknown. We use biochemical, bioinformatics, and genetic techniques to study pathogenesis. Published in PNAS, we showed that 14-3-3z suppresses rheumatoid arthritis. Furthermore, we designed an immunization-based strategy that delays and suppresses rheumatoid (inflammatory) arthritis in animals. In addition to several publications, this project has led to two full patent applications. Ongoing studies focus on translating immunization strategy to human studies.

B) Cytokine Signaling

We examine how 14-3-3z regulates pro-inflammatory cytokines. In particular, we study interleukin-17A and RANKL signal transduction to study the pathogenesis of immune dysfunction. Published in PNAS and JBC, we showed that the adaptor function of 14-3-3z regulates inflammatory bone diseases. Current projects dig deeper into the mechanism and identify targets for immune modulation that will have therapeutic utility.

Lab Team

Chakravarti lab

Publications

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  1. Ayyasamy, S. Fan, P. Czernik, B. Lecka Czernik, S. Chattopadhyay, & R. Chakravarti. 2024.14-3-3z Suppresses Osteoclast Differentiation by Destabilizing TRAF6. Journal of Biological Chemistry.300 (7): 107487
  2. Lughmani, H. Patel, & R. Chakravarti. 2024. Structural features and physiological associations of human 14-3-3z pseudogenes. Genes. 15(4): 399-415.
  3. Fan, S. Popli, S. Chakravarty, R. Chakravarti, & S. Chattopadhyay. 2024. Non-transcriptional IRF7 interacts with NF-kB to inhibit viral inflammation. J Biol Chem. e107200.
  4. Bekbolsynov, A. Waack, C. Buskey, S. Bhadkamkar, K. Rengel, W. Peterson, M.L. Brown, T. Sparkle, D. Kaw, F.J. Syed, S. Chattopadhyay, R. Chakravarti, S. Khuder, B. Mierzejewska, M. Rees, and S. Stepkowski. 2024. Differences in responses of immunosuppressed kidney transplant patients to Moderna mRNA-1273 versus PfizerBioNTech. Vaccines (Basel). 12(1): 91.
  1. Z. Bohrerova, N.E. Brinkman, Chakravarti, S. Chattopadhyay, S.A. Faith, J. Garland, J. Herrin, N. Hull, M. Jahne, D.W. Kang, S.P. Keely, J. Lee, S. Lemeshow, J. Lenhart, E. Lytmer, D. Malgave, L. Miao, A. Minard-Smith, X. Mou, M. Nagarkar, A. Quintero, F.D.R. Savona, J. Senko, J.L. Slonczewski, R.R. Spurbeck, M.G. Sovic, R.T. Taylor, L.K. Weavers, M. Weir. Ohio Coronavirus Wastewater Monitoring Network: Implementation of statewide monitoring for protecting public health. 2023. J. Public Health Manag Pract. 29(6): 845-853.
  2. Chakravarty, G. Subramanian, S. Popli, M. Veleeparambil, S. Fan, R. Chakravarti, & S. Chattopadhyay. 2023. Interferon-stimulated gene TDRD7 interacts with AMPK and inhibits its activation to suppress viral replication and pathogenesis. mBio 14(5): e0061123
  3. Chakravarty, R. Chakravarti, & S. Chattopadhyay. 2023. Inflammatory control of viral infection. Viruses, 15(7): 1529.
  4. Popli, S. Chakravarty, S. Fan, A. Glanz, S. Aras, L. Nagy, G. Sen, R. Chakravarti & S. Chattopadhyay. 2022. IRF3 inhibits nuclear translocation of NF-魏B to prevent viral inflammation. Proceedings of National Academy of Science, 119 (37): e2121385119.
  5. McGowan, M. Borucki, H. Omairi, M. Varghese, S. Vellani S, S. Chakravarty, S. Fan, S. Chattopadhyay, M. Sidiquee, J.B. Thissen, N. Mulakken, J. Moon, J. Kimbrel, A.K. Tiwari, R.T. Taylor, D.W. Kang*, C. Jaing*, R. Chakravarti* & S. Chattopadhyay*. 2022. SARS-CoV-2 monitoring in wastewater reveals novel variants and biomarkers of infection. Viruses, 14 (9): 2032. * co-corresponding author
  6. Chakravarty, S. Fan, R. Chakravarti & S. Chattopadhyay. 2022. Autophagic check and balances of cellular immune responses. Autophagy Reports, 1 (1): 83-87.
  7. Chawla, G. Subramanian, T. Rahman, S. Fan, S. Chakravarty, S. Gujja, H. Demchak, R. Chakravarti & S. Chattopadhyay. 2022. Autophagy in virus infection: A race between host immune response and viral antagonism. Immuno, 2 (1): 153-169.
  8. Okonta, X. Cheng, R. Chakravarti & J. Duggan. 2022. Effects of antibiotic treatment on the microbiota, viral transmission, and viral pathogenesis of MoMuLV ts1 infected BALB/c mice. PLoS One, 17(1): e0261689.
  9. Chakravarti*. 2021. Immune Regulations by 14-3-3: A Misty Terrain. Immunobiology 226(6):152145.
  10. Glanz, S. Chakravarty, S. Fan, K. Chawla, G.S. Subramanian, T. Rahman, D. Walters, R. Chakravarti & S. Chattopadhyay. 2021. Autophagic degradation of IRF3 induced by the small molecule auranofin inhibits transcriptional and pro-apoptotic activities. Journal of Biological Chemistry, 297(5):101274.
  11. Kim, K. Chun, J. McGowan, Y. Zhang, P.J. Czernik, B. Mell, B. Joe, S. Chattopadhyay, J. Holoshitz & R. Chakravarti*. 2021. 14-3-3z: A novel suppressor of inflammatory arthritis. Proceedings of National Academy of Science, 118(34): e2025257118.

(Highlighted in BBC World News and more than 60 national and international news websites)

  1. Glanz, S. Chakravarty, M. Varghese, A. Kottapalli, S. Fan, R. Chakravarti & S. Chattopadhyay. 2021. Transcriptional and non-transcriptional activation, posttranslational modifications, and anti-viral functions of Interferon regulatory factor-3 and viral antagonism by the SARS-Coronavirus. Viruses, 13(4): 575.
  2. M. Ashraf, A.A. Abokor, J.M. Edwards, E.W. Waigi, R.S. Royfman, S.M. Hasan, K.B. Smedlund, A.M.G. Hardy, R. Chakravarti & L.G. Koch. 2021. SARS-Cov2, ACE2 expression, and systemic organ invasion. Physiological Genomics, 53(2):51-60.
  3. McGowan, C. Peter, J. Kim, S. Popli, B. Veerman, J. Saul-Beth, H. Conti, S. Pruett-Miller, S. Chattopadhyay & R. Chakravarti*. 2020. 14-3-3z-TRAF5 axis governs Interleukin-17A signaling. Proceedings of National Academy of Science, 117(40): 25008-250017.
  4. S. Subramanian, S. Popli., S. Chakraborty, R.T. Taylor, R. Chakravarti & S. Chattopadhyay. 2020. Interferon-inducible protein TDRD7 inhibits AMPK to restrict autophagy-independent virus replication. Journal of Biological Chemistry, 295(20):6811-6822.
  5. Glanz, K. Chawla, S. Fabry, G.S. Subramanian, J. Garcia, B. Jay, J. Ciricillo, R. Chakravarti, R.T. Taylor & S. Chattopadhyay. 2020. High throughput screening of FDA-approved drug library reveals the compounds that promote IRF3-mediated proapoptotic pathway inhibit virus replication. Viruses, 12(4): E442.
  6. Benhuri, A. ElJack, B. Kahaleh & R. Chakravarti*. 2020. Mechanisms and biomarkers in aortitis- A Review. Journal of Molecular Medicine (Berl), 98(1): 11-23.
  7. McGowan, C. Peter, S. Chattopadhyay & R. Chakravarti*. 2019. 14-3-3z: A novel immunogen promotes inflammatory cytokine production. Frontiers in Immunology, 10: 1553.
  8. S. Subramanian, R. Chakravarti & S. Chattopadhyay. 2018. Ifi204/p204, a new piece in the sepsis puzzle. Annals of Translational Medicine, 6(1): S12.
  9. A. Sweeny, A.B. Singh, R. Chakravarti, O. Martinez-Guzman, A. Saini, M. Haque, G. Garee, P.D. Dans, L. Hannibal, A.R. Reddi & D.J. Stuehr. 2018. GAPDH is a chaperone that allocates labile heme in cells. Journal of Biological Chemistry, 293(37):14557-14568.
  10. K. Majors, R. Chakravarti, L.M. Ruple, R. Leahy, D.J. Stuehr, M. Lauer, S.C. Erzurum, A. Janocha & M.A. Aronica. 2018. Nitric oxide alters hyaluronan deposition by airway smooth muscle cells. PLoS One, 13(7): e0200074.
  11. S. Subramanian, T. Kuzmanovic, Y. Zhang, C.B. Peter, M. Veleeparambil, R. Chakravarti, G.C. Sen & S. Chattopadhyay. 2018. A New mechanism of interferon鈥檚 antiviral action: Induction of autophagy, essential for paramyxovirus replication, is inhibited by the interferon-stimulated gene, TDRD7. PLoS Pathogens, 14(1): e1006877.
  1. McGowan, J. Kratch, S. Chattopadhyay, B. Joe, H. Conti & R. Chakravarti* 2017. Bioinformatic analysis reveals new determinants of antigenic 14-3-3 proteins and a novel antifungal strategy. PLoS One, 12(12): e0189503.
  2. Veerman & R. Chakravarti*. 2017. Qualitative and Quantitative assay of circulating autoantibodies against human tissue antigen. Bio-Protocol, 7(13).
  3. Swain, A. Bosali, G.S. Hoffman & R. Chakravarti*. 2017. 14-3-3: A glue for medial inflammation. Journal of Investigative Medicine, 65(4):874-875.
  4. S. Subrammanian, K. Pan, R. Chakravarti, & S. Chattopadhyay. 2016. Biochemical Analysis of Caspase-8-dependent Proteolysis of IRF-3 in Virus-infected Cells. Bioprotocol, 6(22).
  5. Chakravarti*, K. Gupta, M. Swain, B. Willard, J. Scholtz, L. Svensson, E. Roselli, G. Pettersson, D. Johnston, E. Soltesz, M. Yamashita, D. Stuehr, T.M. Daly & G.S. Hoffman. 2015. 14-3-3 in aortic aneurysms: Identification of a novel auto-antigen in large vessel vasculitis. Arthritis & Rheumatology, 67(7):1913-1921.
  6. Chakravarti, K. Gupta, A. Majors, L. Ruple, M. Aronica & D. Stuehr. 2015. Novel insights in mammalian catalase heme maturation: Effect of NO and thioredoxin-1. Free Radical Biology & Medicine, 82: 105-113.
  7. Zhang, J. Amick, R. Chakravarti, S. Santarriaga, S. Schlanger, C. McGlone, M. Dare, J.C. Nix, K.M. Scagline, D.J. Stuehr, S. Misra & R.C. Page. 2015. A bipartite interaction between Hsp70 and CHIP regulates ubiquitination of chaperoned client proteins. Structure, 23(3): 472-482.
  8. Revsbech, X. Shen, R. Chakravarti, F.B. Jensen, B. Thiel, A.L. Evans, J. Kindberg, O. Fr枚bert, D.J. Stuehr, C.G. Kevil & A. Fago. 2014. Hydrogen sulfide and nitric oxide metabolites in the blood of free-ranging brown bears and their potential roles in hibernation. Free Radical Biology & Medicine, 73: 349-357.
  9. Chakravarti & D.J. Stuehr. 2012. Thioredoxin regulates cellular heme insertion via denitrosylation of Glyceraldehyde-3-phosphate dehydrogenase. Journal of Biological Chemistry, 287(20): 16179-16186.
  10. Hannibal, D. Collins, J. Brassard, R. Chakravarti, R. Vempati, P. Dorlet, J. Santolini, J.H. Dawson & D.J. Stuehr. 2012. Heme binding properties of Glyceraldehyde-3-phosphate Dehydrogenase. Biochemistry, 51(43): 8514-8529.
  11. Aytekin, K.S Aulak, Haserodt S, R. Chakravarti, J. Cody, O.A. Minai & R.A. Dweik. 2012. Abnormal platelet aggregation in idiopathic pulmonary arterial hypertension: Role of nitric oxide. American Journal of Physiology- Lung Cellular & Molecular Physiology, 302(6): L512-520.
  12. Chakravarti, K.S. Aulak, P.L. Fox & D.J. Stuehr. 2010. GAPDH regulates cellular heme insertion into inducible nitric oxide synthase. Proceedings of National Academy of Science, 107(42): 18004-18009.

(Commentary in 鈥楩aculty of 1000鈥� under the exceptional category)

  1. M. Waheed, A. Ghosh, R. Chakravarti, A. Biswas, M.M. Haque, K. Panda & D.J. Stuehr. 2010. Nitric oxide blocks cellular heme insertion into a broad range of heme proteins. Free Radical Biology & Medicine, 48: 1548-1558.
  2. Valiyaveettil, A. Bentley, P. Gurusahaney, R. Hussien, R. Chakravarti, N. Kureishy, S. Prag & J.C. Adams. 2008. Novel role of muskelin/RanBP9 Complex as a nucleocytoplasmic mediator of cell morphology regulation. Journal of Cell Biology, 182(4):727-739.
  3. Chakravarti & J.C. Adams. 2006. Comparative genomics of the syndecans defines an ancestral genomic context associated with matrilins in vertebrates. BMC Genomics, 7(1): 83.
  4. Chakravarti, V. Sapountzi & J.C. Adams. 2005. Functional role of syndecan-1 cytoplasmic V region in lamellipodial spreading, actin bundling, and cell migration. Molecular Biology of the Cell, 16(8): 3678-3691.
  5. Chakravarti & V. Sahai. 2004. Compactin - A review. Applied Microbiology & Biotechnology, 64(5): 618-624.
  6. Chakravarti & V. Sahai. 2002. Optimization of compactin production in chemically defined production medium by Penicillium citrinum Using Statistical Methods. Process Biochemistry, 38(4): 481-486.
  7. Chakravarti & V. Sahai. 2002. A new medium for the production of compactin by Penicillium citrinum. Biotechnology Letters, 24: 527-530.

Patents

Novel role of 14-3-3 inhibitor as an anti-fungal agent.
Treatment of Rheumatoid Arthritis by Therapeutic Administration of 14-3-3zeta.

Academic Appointments

  • Assistant Professor, Department of Physiology and Pharmacology, University of Toledo, College of Medicine and Life Sciences, Toledo, OH. 2018-present
  • Assistant Professor, Department of Surgery, University of Toledo, College of Medicine and Life Sciences, Toledo, OH. 2016-2018
  • Assistant Professor, Cleveland Clinic Lerner College of Medicine, Cleveland, OH. 2014-2016
  • Joint Staff, Rheumatic and Immunological Disease, Cleveland Clinic, Cleveland, OH. 2014-2016
  • Project Staff, Lerner Research Institute, Cleveland Clinic, Cleveland, OH. 2013- 2016
  • Research Associate, Lerner Research Institute, Cleveland, OH. 2006-2013
  • Post-Doctoral Fellow, Lerner Research Institute, Cleveland Clinic, Cleveland, OH. 2002-2012

Awards and honors

  • Early Career Faculty Travel Award 2019, Immunology 2019, San Diego, CA, USA.
  • CSCTR Travel Award 2017, Central Society of Clinical & Translational Research, Chicago, USA.
  • Travel Award 2011, Gordon Research Conference 鈥楴itric Oxide鈥�, Ventura, California, USA.
  • Travel Award 2010, The 6th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide鈥�, Kyoto, Japan.
  • Moran Fellowship in Cardiovascular Research, Department of Cell Biology, Lerner Research Institute (15 Mar 2006 - Dec 2006)
  • Elsa Albrecht Fellow Award 2005, Department of Cell Biology, Lerner Research Institute, USA
  • Young Histochemist Award 2004, International Federation of Societies for Histochemistry and Cytochemistry, USA
  • National Educational Talent Fellowship, Council of Scientific and Industrial Research, India (1998-2002)
  • Common Entrance Test Fellowship, Department of Biotechnology, India (1995-1997)

Education

Ph.D., Indian Institute of Technology-Delhi, India